Effect of Quercetin on Paraoxonase 2 Levels in RAW264.7 Macrophages and in Human Monocytes-Role of Quercetin Metabolism

Boesch-Saadatmandi, C., Pospissil, R. T., Graeser, A. C., Canali, R., Boomgaarden, I., Doering, F., Wolffram, S., Egert, S., Mueller, M. J. and Rimbach, Gerald (2009) Effect of Quercetin on Paraoxonase 2 Levels in RAW264.7 Macrophages and in Human Monocytes-Role of Quercetin Metabolism International Journal of Molecular Sciences, 10 (9). pp. 4168-4177.

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Abstract

There is increasing evidence that the intracellular antioxidant enzyme paraoxonase 2 (PON2) may have a protective function in the prevention of atherogenesis. An enhancement of PON2 activity by dietary factors including flavonoids is therefore of interest. In the present study we determined the effect of quercetin on paraoxonase 2 levels in cultured murine macrophages in vitro and in overweight subjects with a high cardiovascular risk phenotype supplemented with 150 mg quercetin/day for 42 days in vivo. Supplementation of murine RAW264.7 macrophages in culture with increasing concentrations of quercetin (1, 10, 20 mu mol/L) resulted in a significant increase in PON2 mRNA and protein levels, as compared to untreated controls. Unlike quercetin, its glucuronidated metabolite quercetin-3-glucuronide did not affect PON2 gene expression in cultured macrophages. However the methylated quercetin derivative isorhamnetin enhanced PON2 gene expression in RAW264.7 cells to similar extent like quercetin. Although supplementing human volunteers with quercetin was accompanied by a significant increase in plasma quercetin concentration, dietary quercetin supplementation did not change PON2 mRNA levels in human monocytes in vivo. Current data indicate that quercetin supplementation increases PON2 levels in cultured monocytes in vitro but not in human volunteers in vivo.

Document Type: Article
Keywords: quercetinflavonoid paraoxonase 2 macrophage atherosclerosis low-density-lipoprotein oxidative stress antioxidant properties pon2 expression plasma atherosclerosis activation mice absorption flavonoids
Research affiliation: Kiel University
OceanRep > The Future Ocean - Cluster of Excellence
Refereed: Yes
ISSN: 1422-0067
Projects: Future Ocean
Date Deposited: 11 Feb 2011 12:13
Last Modified: 23 May 2017 10:20
URI: http://eprints.uni-kiel.de/id/eprint/9299

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