Methane- and ammonia-oxidizing bacteria at the chemocline of Lake Kinneret (Israel)

Junier, Pilar, Kim, Ok-Sun, Eckert, Werner, Casper, Peter, Imhoff, Johannes F., Witzel, Karl-Paul and Hadas, Ora (2010) Methane- and ammonia-oxidizing bacteria at the chemocline of Lake Kinneret (Israel) Aquatic Microbial Ecology, 58 . pp. 241-248. DOI 10.3354/ame01373.

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Abstract

The vertical distribution of methane- and ammonia-oxidizing bacteria (MOB and AOB, respectively), and the physicochemical conditions in the chemocline of Lake Kinneret (Israel) were studied at a resolution of 10 cm from 16.2 to 17.7 m depth. Profiles of the chemical parameters indicated decreasing concentrations of methane (from 22.4 to 0.11 µmol l–1) and ammonia (from 14.2 to 8.4 µmol l–1) towards the water surface and in close proximity to the chemocline. The disappearance of methane coincided with methane oxidation that could be corroborated throughout this layer with highest rates at 17.4 to 17.6 m. Disappearance of ammonia could not be linked to ammonia oxidation exclusively. The genes pmoA and the homologous amoA (coding for subunit α of the methane and ammonia monooxygenase, respectively) were amplified by PCR. The products were analyzed by terminal restriction fragment length polymorphism (T-RFLP) and sequencing of clone libraries. The results demonstrated that different MOB and AOB communities are established along the concentration gradient within the narrow layer of the metalimnetic chemocline. Changes in the intensity of the T-RFLP peaks and the frequency of different groups of alpha- and gammaproteobacterial MOB, and betaproteobacterial AOB, coincided with the concentration gradients of methane, ammonia, nitrate, and oxygen in the chemocline. This suggests that different communities of MOB, and to a lesser extent AOB, contribute to the formation of chemical gradients of their particular substrates in the chemocline

Document Type: Article
Keywords: Microbiology; amoA; pmoA; Chemocline; Ammonia; Methane
Research affiliation: OceanRep > GEOMAR > FB3 Marine Ecology > FB3-MI Marine Microbiology
OceanRep > The Future Ocean - Cluster of Excellence
Refereed: Yes
DOI etc.: 10.3354/ame01373
ISSN: 0948-3055
Projects: Future Ocean
Date Deposited: 06 Aug 2010 09:38
Last Modified: 26 May 2016 09:28
URI: http://eprints.uni-kiel.de/id/eprint/8759

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