HPLC Determination and Pharmacokinetic Study of Homoeriodictyol-7-O-â- D- glucopyranoside in Rat Plasma and Tissues

Yunli, Z., Xiaoying, W., Yingchun, Z., Xiaoxia, G., Kaishun, B. and Zhiguo, Yu (2007) HPLC Determination and Pharmacokinetic Study of Homoeriodictyol-7-O-â- D- glucopyranoside in Rat Plasma and Tissues Biological & Pharmaceutical Bulletin, 30 (4). pp. 617-620. DOI 10.1248/bpb.30.617.

[img]
Preview
Text
30_617.pdf - Published Version
Available under License ["licenses_description_cc_by_3.0" not defined].

Download (117Kb) | Preview

Supplementary data:

Abstract

Homoeriodictyol-7-O-β-D-glucopyranoside (HEDT-Glu) was isolated from Viscum coloratum and identified by MS, 1H- and 13C-NMR. A HPLC method was developed for determination of HEDT-Glu in rat plasma and tissues. All biological samples were pretreated by protein precipitation with acetone. Vanillin was selected as internal standard. The mobile phase consisted of methanol–water–glacial acetic acid (45 : 55 : 0.5, v/v/v). Good linearity were observed over the concentration ranges of 0.1—200.0 μg·ml−1 in rat plasma and 0.05—5.0 μg·ml−1 in tissues. Both intra- and inter-day precisions of HEDT-Glu, expressed as the relative standard deviation, were less than 13.1%. Accuracy, expressed as the relative error, ranged from −0.8 to 5.4% in plasma and from −5.6 to 9.4% in tissues. The mean extraction recovery of HEDT-Glu was above 73.17% in biological samples. The described assay method was successfully applied to the pre-clinical pharmacokinetic study of HEDT-Glu. After intravenous administration of HEDT-Glu to rat, AUC and CLtot were 16.04±3.19 μg·h·ml−1 and 0.85±0.17 l·kg−1·h−1, respectively. T1/2,α and t1/2,β were 0.06±0.01 h and 1.27±0.31 h, respectively. HEDT-Glu was cleared from the blood and mainly distributed to the liver and small intestine.

Document Type: Article
Keywords: homoeriodictyol-7-O-β-D-glucopyranoside, Viscum coloratum, rat plasma, tissue, HPLC determination, pharmacokinetics
Research affiliation: OceanRep > GEOMAR > FB3 Marine Ecology > FB3-MI Marine Microbiology
Refereed: Yes
DOI etc.: 10.1248/bpb.30.617
ISSN: 0918-6158
Date Deposited: 03 Dec 2008 16:51
Last Modified: 17 Jun 2016 10:38
URI: http://eprints.uni-kiel.de/id/eprint/8150

Actions (login required)

View Item View Item

Document Downloads

More statistics for this item...