Bch-Y-based degenerate primers target all types of anoxygenic photosynthetic bacteria in a single PCR reaction

Yutin, N., Rosenberg, M., Rotem, D., Suzuki, M. T., Madigan, M. T., Süling, Jörg, Imhoff, Johannes F., Beja, O., Zohary, T., Eckert, W. and Yacobi, Y. Z. (2009) Bch-Y-based degenerate primers target all types of anoxygenic photosynthetic bacteria in a single PCR reaction Applied and Environmental Microbiology, 75 . pp. 7556-7559. DOI 10.1128/AEM.01014-09.

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Abstract

To detect anoxygenic bacteria containing either type 1 or type 2 photosynthetic reaction centers in a single PCR, we designed a degenerate primer set based on the bchY gene. The new primers were validated in silico using the GenBank nucleotide database as well as by PCR on pure strains and environmental DNA.

Document Type: Article
Research affiliation: OceanRep > GEOMAR > FB3 Marine Ecology > FB3-MI Marine Microbiology
OceanRep > The Future Ocean - Cluster of Excellence
Kiel University
Refereed: Yes
DOI etc.: 10.1128/AEM.01014-09
ISSN: 0099-2240
Projects: Future Ocean
Date Deposited: 10 Nov 2009 12:34
Last Modified: 26 Apr 2016 12:16
URI: http://eprints.uni-kiel.de/id/eprint/7545

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