Molecular techniques and their limitations shape our view of the holobiont

Cooke, Ira, Mead, Oliver, Whalen, Casey, Boote, Chloë, Moya, Aurelie, Ying, Hua, Robbins, Steven, Strugnell, Jan M., Darling, Aaron, Miller, David, Voolstra, Christian R., Adamska, Maja, Ainsworth, Tracy, Andrade, Natalia R., Arnold, A. Elizabeth, Ball, Eldon, Bourne, David, Bosch, Thomas, Butterfield, Nicholas J., Chan, Cheong Xin, Cowman, Peter E., Davy, Simon K., Fabricius, Katharina, Fortunato, Sofia V., Fraune, Sebastian, Hernandez, Alejandra, Hoogenboom, Mia, Jaspers, Cornelia, Mohamed, Amin, Pita, Lucia, Ragan, Mark A., Sakamaki, Kazuhiro, Schoepf, Verena, Seemann, Torsten, Shinzato, Chuya, Stolarski, Jaroslaw, Takahashi, Shunichi, Tang, Sen-Lin, Webster, Nicole and Whitelaw, Brooke (2019) Molecular techniques and their limitations shape our view of the holobiont Zoology, 137 . Art.Nr. 125695. DOI 10.1016/j.zool.2019.125695.

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It is now recognised that the biology of almost any organism cannot be fully understood without recognising the existence and potential functional importance of associated microbes. Arguably, the emergence of this holistic viewpoint may never have occurred without the development of a crucial molecular technique, 16S rDNA amplicon sequencing, which allowed microbial communities to be easily profiled across a broad range of contexts. A diverse array of molecular techniques are now used to profile microbial communities, infer their evolutionary histories, visualise them in host tissues, and measure their molecular activity. In this review, we examine each of these categories of measurement and inference with a focus on the questions they make tractable, and the degree to which their capabilities and limitations shape our view of the holobiont.

Document Type: Article
Additional Information: Consortium of Australian Academy of Science Boden Research Conference Participants: Ainsworth et.seq.
Keywords: Holobiont; Imaging; Metagenomic sequencing; Evolutionary inference; Model system; Multi-omics
Research affiliation: OceanRep > GEOMAR > FB3 Marine Ecology > FB3-EV Marine Evolutionary Ecology
OceanRep > GEOMAR > FB3 Marine Ecology > FB3-MS Marine Symbioses
Kiel University
Refereed: Yes
DOI etc.: 10.1016/j.zool.2019.125695
ISSN: 0944-2006
Projects: SFB1182
Date Deposited: 07 Feb 2020 08:04
Last Modified: 10 Feb 2020 13:10

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