Host-Microbe Interactions in the Chemosynthetic Riftia pachyptila Symbiosis

Hinzke, Tjorven, Kleiner, Manuel, Breusing, Corinna, Felbeck, Horst, Häsler, Robert, Sievert, Stefan M., Schlüter, Rabea, Rosenstiel, Philip, Reusch, Thorsten B. H., Schweder, Thomas, Markert, Stephanie, Distel, Daniel and Ruby, Edward G. (2019) Host-Microbe Interactions in the Chemosynthetic Riftia pachyptila Symbiosis mBio, 10 (6). e02243-19. DOI 10.1128/mBio.02243-19.

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The deep-sea tubeworm Riftia pachyptila lacks a digestive system but completely relies on bacterial endosymbionts for nutrition. Although the symbiont has been studied in detail on the molecular level, such analyses were unavailable for the animal host, because sequence information was lacking. To identify host-symbiont interaction mechanisms, we therefore sequenced the Riftia transcriptome, which served as a basis for comparative metaproteomic analyses of symbiont-containing versus symbiont-free tissues, both under energy-rich and energy-limited conditions. Our results suggest that metabolic interactions include nutrient allocation from symbiont to host by symbiont digestion and substrate transfer to the symbiont by abundant host proteins. We furthermore propose that Riftia maintains its symbiont by protecting the bacteria from oxidative damage while also exerting symbiont population control. Eukaryote-like symbiont proteins might facilitate intracellular symbiont persistence. Energy limitation apparently leads to reduced symbiont biomass and increased symbiont digestion. Our study provides unprecedented insights into host-microbe interactions that shape this highly efficient symbiosis.

Document Type: Article
Keywords: host-microbe interactions, symbiosis, holobiont, chemosynthesis, hydrothermal vents, metaproteomics
Research affiliation: OceanRep > GEOMAR > FB3 Marine Ecology > FB3-EV Marine Evolutionary Ecology
Refereed: Yes
DOI etc.: 10.1128/mBio.02243-19
ISSN: 2150-7511
Projects: SFB 1182
Date Deposited: 06 Jan 2020 08:19
Last Modified: 09 Jan 2020 12:11

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