Proteomic investigation of the blue mussel larval shell organic matrix

Carini, Alessia, Koudelka, Tomas, Tholey, Andreas, Appel, Esther, Gorb, Stanislav N., Melzner, Frank and Ramesh, Kirti (In Press / Accepted) Proteomic investigation of the blue mussel larval shell organic matrix Journal of Structural Biology . DOI 10.1016/j.jsb.2019.09.002.

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Abstract

Shell matrix proteins (SMPs) are occluded within molluscan shells and are fundamental to the biological control over mineralization. While many studies have been performed on adult SMPs, those of larval stages remain largely undescribed. Therefore, this study aimed to characterize the larval shell proteome of the blue mussel for the first time and to compare it to adult mussel shell proteomes. Following development of a method for cleaning larval shells of tissue contaminants, 49 SMPs were identified using shotgun proteomics. Twenty-one proteins were independently identified in all samples indicating that they form a subset of the core larval shell proteome. These included: the blue mussel shell protein, a peroxidase domain-containing sequence, a laminin G domain-containing sequence, a ZIP domain-containing sequence and a ferric-chelate reductase 1-like sequence. Additional SMP domains identified were: fibronectin type III, BPTI/Kunitz, chitin-binding type 3, thyroglobulin and EF-hand. While key predictable molluscan shell matrix functions are identified, 67% of sequences remain unknown or uncharacterized, indicating that this shell proteome is unique to mussel larvae. Specifically, comparison with adult mytilids reveals that nine domains are exclusive to the larval shell proteome and only four domains are conserved among species and developmental stages. Thus, strong species-specific and ontogenetic variation exists in shell proteome composition.

Document Type: Article
Keywords: Biomineralization; Mussel larvae; Organic matrix; Shell matrix protein
Research affiliation: Kiel University > Kiel Marine Science
OceanRep > The Future Ocean - Cluster of Excellence
Kiel University
OceanRep > GEOMAR > FB3 Marine Ecology > FB3-EOE-B Experimental Ecology - Benthic Ecology
Refereed: Yes
DOI etc.: 10.1016/j.jsb.2019.09.002
ISSN: 1047-8477
Projects: Future Ocean, CACHE
Date Deposited: 23 Oct 2019 08:59
Last Modified: 23 Oct 2019 08:59
URI: http://eprints.uni-kiel.de/id/eprint/48058

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