Expression of calcification‐related ion transporters during blue mussel larval development

Ramesh, Kirti, Yarra, Tejaswi, Clark, Melody S., John, Uwe and Melzner, Frank (2019) Expression of calcification‐related ion transporters during blue mussel larval development Ecology and Evolution, 9 (12). pp. 7157-7172. DOI 10.1002/ece3.5287.

[img]
Preview
Text
Ramesh_et_al-2019-Ecology_and_Evolution.pdf - Published Version
Available under License Creative Commons: Attribution 4.0.

Download (800Kb) | Preview

Supplementary data:

Abstract

The physiological processes driving the rapid rates of calcification in larval bivalves are poorly understood. Here, we use a calcification substrate-limited approach (low dissolved inorganic carbon, CT) and mRNA sequencing to identify proteins involved in bicarbonate acquisition during shell formation. As a secondary approach, we examined expression of ion transport and shell matrix proteins (SMPs) over the course of larval development and shell formation. We reared four families of Mytilus edulis under ambient (ca. 1865 µmol/kg) and low CT (ca. 941 µmol/kg) conditions and compared expression patterns at six developmental time points. Larvae reared under low CT exhibited a developmental delay, and a small subset of contigs was differentially regulated between ambient and low CT conditions. Of particular note was the identification of one contig encoding an anion transporter (SLC26) which was strongly upregulated (2.3–2.9 fold) under low CT conditions. By analyzing gene expression profiles over the course of larval development, we are able to isolate sequences encoding ion transport and SMPs to enhance our understanding of cellular pathways underlying larval calcification processes. In particular, we observe the differential expression of contigs encoding SLC4 family members (sodium bicarbonate cotransporters, anion exchangers), calcium-transporting ATPases, sodium/calcium exchangers, and SMPs such as nacrein, tyrosinase, and transcripts related to chitin production. With a range of candidate genes, this work identifies ion transport pathways in bivalve larvae and by applying comparative genomics to investigate temporal expression patterns, provides a foundation for further studies to functionally characterize the proteins involved in larval calcification.

Document Type: Article
Additional Information: Data Availability Statement:: Transcriptomics data are available at the NCBI short read archive under accession number PRJNA494236
Keywords: biomineralization, bivalves, gene expression, larvae
Research affiliation: HIFMB
AWI
OceanRep > GEOMAR > FB3 Marine Ecology > FB3-EOE-B Experimental Ecology - Benthic Ecology
Refereed: Yes
DOI etc.: 10.1002/ece3.5287
ISSN: 2045-7758
Related URLs:
Projects: CACHE, REA, PACES-II
Date Deposited: 13 Aug 2019 08:32
Last Modified: 13 Aug 2019 08:33
URI: http://eprints.uni-kiel.de/id/eprint/47460

Actions (login required)

View Item View Item

Document Downloads

More statistics for this item...