Seeking active RubisCOs from the currently uncultured microbial majority colonizing deep-sea hydrothermal vent environments

Böhnke, Stefanie and Perner, Mirjam (2019) Seeking active RubisCOs from the currently uncultured microbial majority colonizing deep-sea hydrothermal vent environments The ISME Journal . DOI 10.1038/s41396-019-0439-3.

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Abstract

Almost all the inorganic carbon on Earth is converted into biomass via the Calvin–Benson–Bassham (CBB) cycle. Here, the central carboxylation reaction is catalyzed by ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO), which can be found in numerous primary producers including plants, algae, cyanobacteria, and many autotrophic bacteria. Although RubisCO possesses a crucial role in global biomass production, it is not a perfect catalyst. Therefore, research interest persists on accessing the full potential of yet unexplored RubisCOs. We recently developed an activity-based screen suited to seek active recombinant RubisCOs from the environment—independent of the native host’s culturability. Here, we applied this screen to twenty pre-selected genomic fosmid clones from six cultured proteobacteria to demonstrate that a broad range of phylogenetically distinct RubisCOs can be targeted. We then screened 12,500 metagenomic fosmid clones from six distinct hydrothermal vents and identified forty active RubisCOs. Additional sequence-based screening uncovered eight further RubisCOs, which could then also be detected by a modified version of the screen. Seven were active form III RubisCOs from yet uncultured Archaea. This indicates the potential of the activity-based screen to detect RubisCO enzymes even from organisms that would not be expected to be targeted.

Document Type: Article
Research affiliation: OceanRep > GEOMAR > FB2 Marine Biogeochemistry > FB2-MG Marine Geosystems
Refereed: Yes
DOI etc.: 10.1038/s41396-019-0439-3
ISSN: 1751-7362
Projects: SPP 1144 "From Mantle to Ocean"
Expeditions/Models:
Date Deposited: 17 Jun 2019 12:00
Last Modified: 17 Jun 2019 12:07
URI: http://eprints.uni-kiel.de/id/eprint/46941

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