Boron isotope variability related to B speciation (change during uptake and transport) in bell pepper plants and SI traceable n ( 11 B)/ n ( 10 B) ratios for plant reference materials

Geilert, Sonja, Vogl, Jochen, Rosner, Martin and Eichert, Thomas (2019) Boron isotope variability related to B speciation (change during uptake and transport) in bell pepper plants and SI traceable n ( 11 B)/ n ( 10 B) ratios for plant reference materials Rapid Communications in Mass Spectrometry . DOI 10.1002/rcm.8455.

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Abstract

Rationale

Boron (B) is an essential micronutrient in plants and its isotope variations are used to gain insights into plant metabolism, which is important for crop plant cultivation. B isotope variations were used to trace intraplant fractionation mechanisms in response to the B concentration in the irrigation water spanning the range from B depletion to toxic levels.

Methods

A fully validated analytical procedure based on MC‐ICP‐MS, sample decomposition and B matrix separation was applied to study B isotope fractionation. The validation was accomplished by establishing a complete uncertainty budget and by applying reference materials, yielding expanded measurement uncertainties of 0.8 ‰ for pure boric acid solutions and ≤ 1.5 ‰ for processed samples. With this validated procedure SI traceable B isotope amount ratios were determined in plant reference materials for the first time.

Results

The B isotope compositions of irrigation water and bell pepper samples suggest passive diffusion of the heavy 11B isotope into the roots during low to high B concentrations while uptake of the light 10B isotope was promoted during B depletion, probably by active processes. A systematic enrichment of the heavy 11B isotope in higher located plant parts was observed (average Δ11Bleaf‐roots = 20.3 ± 2.8 ‰ (1 SD)), possibly by a facilitated transport of the heavy 11B to growing meristems by B transporters.

Conclusions

B isotopes can be used to identify plant metabolism in response to the B concentration in the irrigation water and during intraplant B transfer. The large B isotope fractionation within the plants demonstrates the importance of biological B cycling for the global B cycle.

Document Type: Article
Research affiliation: OceanRep > GEOMAR > FB2 Marine Biogeochemistry > FB2-MG Marine Geosystems
Refereed: Yes
DOI etc.: 10.1002/rcm.8455
ISSN: 0951-4198
Date Deposited: 24 Apr 2019 12:40
Last Modified: 24 Apr 2019 12:40
URI: http://eprints.uni-kiel.de/id/eprint/46422

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