Antitumor Anthraquinones from an Easter Island Sea Anemone: Animal or Bacterial Origin?

Sottorff, Ignacio, Künzel, Sven, Wiese, Jutta, Lipfert, Matthias, Preußke, Nils, Sönnichsen, Frank and Imhoff, Johannes F. (2019) Antitumor Anthraquinones from an Easter Island Sea Anemone: Animal or Bacterial Origin? Marine Drugs, 17 (3). p. 154. DOI 10.3390/md17030154.

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The presence of two known anthraquinones, Lupinacidin A and Galvaquinone B, which have antitumor activity, has been identified in the sea anemone (Gyractis sesere) from Easter Island. So far, these anthraquinones have been characterized from terrestrial and marine Actinobacteria only. In order to identify the anthraquinones producer, we isolated Actinobacteria associated with the sea anemone and obtained representatives of seven actinobacterial genera. Studies of cultures of these bacteria by HPLC, NMR, and HRLCMS analyses showed that the producer of Lupinacidin A and Galvaquinone B indeed was one of the isolated Actinobacteria. The producer strain, SN26_14.1, was identified as a representative of the genus Verrucosispora. Genome analysis supported the biosynthetic potential to the production of these compounds by this strain. This study adds Verrucosispora as a new genus to the anthraquinone producers, in addition to well-known species of Streptomyces and Micromonospora. By a cultivation-based approach, the responsibility of symbionts of a marine invertebrate for the production of complex natural products found within the animal’s extracts could be demonstrated. This finding re-opens the debate about the producers of secondary metabolites in sea animals. Finally, it provides valuable information about the chemistry of bacteria harbored in the geographically-isolated and almost unstudied, Easter Island

Document Type: Article
Keywords: Easter Island; Actinobacteria; anthraquinones; symbionts; sea anemone; marine invertebrates; spectroscopy; chromatography
Research affiliation: OceanRep > GEOMAR > FB3 Marine Ecology > FB3-MI Marine Microbiology
Refereed: Yes
DOI etc.: 10.3390/md17030154
ISSN: 1660-3397
Date Deposited: 06 Mar 2019 10:50
Last Modified: 06 Mar 2019 10:50

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