Thermal Stimulation of the Retina Reduces Bruch's Membrane Thickness in Age Related Macular Degeneration Mouse Models

Tode, Jan, Richert, Elisabeth, Koinzer, Stefan, Klettner, Alexa, von der Burchard, Claus, Brinkmann, Ralf, Lucius, Ralph and Roider, Johann (2018) Thermal Stimulation of the Retina Reduces Bruch's Membrane Thickness in Age Related Macular Degeneration Mouse Models Translational Vision Science & Technology, 7 (3). p. 2. DOI 10.1167/tvst.7.3.2.

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Supplementary data:

Abstract

Purpose:

To investigate the effect of thermal stimulation of the retina (TS-R) on Bruch's membrane (BrM) thickness in age-related macular degeneration (AMD) mouse models as a novel concept for the prophylaxis and treatment of dry AMD.
Methods:

Two knockout AMD mouse models, B6.129P2-Apoetm1Unc/J (ApoE-/-) and B6.129X1-Nfe2I2tm1Ywk/J (NRF2-/-), were chosen. One randomized eye of each mouse in four different groups (two of different age, two of different genotype) of five mice was treated by TS-R (532 nm, 10-ms duration, 50-μm spot size), the fellow eye served as control. Laser power was titrated to barely visible laser burns, then reduced by 70% to guarantee for thermal elevation without damage to the neuroretina, then applied uniformly to the murine retina. Fundus, optical coherence tomography (OCT), and fluorescein angiography (FLA) images were obtained at the day of treatment and 1 month after treatment. Eyes were enucleated thereafter to analyze BrM thickness by transmission electron microscopy (TEM) in a standardized blinded manner.
Results:

Fundus images revealed that all ApoE-/- and NRF2-/- mice had AMD associated retinal alterations. BrM thickness was increased in untreated controls of both mouse models. Subvisible TS-R laser spots were not detectable by fundus imaging, OCT, or FLA 2 hours or 1 month after laser treatment. TEM revealed a significant reduction of BrM thickness in laser-treated eyes of all four groups compared to their fellow control eyes.
Conclusions:

TS-R reduces BrM thickness in AMD mouse models ApoE-/- and NRF2-/- without damage to the neuroretina. It may become a prophylactic or even therapeutic treatment option for dry AMD.
Translational Relevance:

TS-R may become a prophylactic or even therapeutic treatment option for dry AMD.

Document Type: Article
Keywords: Bruch's membrane; age related macular degeneration (AMD); drusen; nondamaging retinal laser therapy (NRT); subthreshold laser therapy; thermal stimulation of the retina (TS-R)
Research affiliation: Kiel University > Kiel Marine Science
OceanRep > The Future Ocean - Cluster of Excellence
Refereed: Yes
DOI etc.: 10.1167/tvst.7.3.2
ISSN: 2164-2591
Projects: Future Ocean
Date Deposited: 06 Dec 2018 10:35
Last Modified: 06 Dec 2018 10:36
URI: http://eprints.uni-kiel.de/id/eprint/44856

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