Predicted bacterial interactions affect in vivo microbial colonization dynamics in Nematostella

Domin, Hanna, Zurita-Gutierrez, Yazmín H., Scotti, Marco, Buttlar, Jann, Hentschel Humeida, Ute and Fraune, Sebastian (2018) Predicted bacterial interactions affect in vivo microbial colonization dynamics in Nematostella Frontiers in Microbiology, 9 . Art.Nr. 728. DOI 10.3389/fmicb.2018.00728.

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Abstract

The maintenance and resilience of host-associated microbiota during development is a fundamental process influencing the fitness of many organisms. Several host properties were identified as influencing factors on bacterial colonization, including the innate immune system, mucus composition and diet. In contrast, the importance of bacteria-bacteria interactions on host colonization is less understood. Here, we use bacterial abundance data of the marine model organism Nematostella vectensis to reconstruct potential bacteria-bacteria interactions through co-occurrence networks. The analysis indicates that bacteria-bacteria interactions are dynamic during host colonization and change according to the host’s developmental stage. To assess the predictive power of inferred interactions, we tested bacterial isolates with predicted cooperative or competitive behavior for their ability to influence bacterial recolonization dynamics. Within three days of recolonization all tested bacterial isolates affected bacterial community structure, while only competitive bacteria increased bacterial diversity. Already one week after recolonization almost no differences in bacterial community structure could be observed between control and treatments. These results show that predicted competitive bacteria can influence community structure for a short period of time, verifying the in silico predictions. However, within one week, the effects of the bacterial isolates are neutralized, indicating a high degree of resilience of the bacterial community.

Hanna Domin1, Yazmín H. Zurita-Gutiérrez2, Marco Scotti3, Jann Buttlar1, Ute Hentschel Humeida2 and Sebastian Fraune1*

Document Type: Article
Keywords: correlation networks, Bacteria-bacteria interactions, holobiont, Host-Microbe Interactions, Cnidaria, metaorganism, resilience, community ecology
Research affiliation: OceanRep > GEOMAR > FB3 Marine Ecology > FB3-EOE-N Experimental Ecology - Food Webs
Kiel University > Kiel Marine Science
OceanRep > GEOMAR > FB3 Marine Ecology > FB3-MI Marine Microbiology
OceanRep > The Future Ocean - Cluster of Excellence
Kiel University
Refereed: Yes
DOI etc.: 10.3389/fmicb.2018.00728
ISSN: 1664-302X
Projects: Future Ocean
Date Deposited: 04 Apr 2018 10:59
Last Modified: 24 Apr 2018 09:29
URI: http://eprints.uni-kiel.de/id/eprint/42552

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