First genetic quantification of sex- and stage-specific feeding in the ubiquitous copepod Acartia tonsa

Ismar, Stefanie M. H., Kottmann, Johanna S. and Sommer, Ulrich (2018) First genetic quantification of sex- and stage-specific feeding in the ubiquitous copepod Acartia tonsa Marine Biology, 165 (2). Art.No. 25. DOI 10.1007/s00227-017-3281-z.

[img] Text
10.1007_s00227-017-3281-z.pdf - Published Version
Restricted to Registered users only

Download (1099Kb) | Contact
[img] Text
227_2017_3281_MOESM1_ESM.pdf - Supplemental Material
Restricted to Registered users only

Download (798Kb) | Contact
[img] Text
227_2017_3281_MOESM2_ESM.pdf - Supplemental Material
Restricted to Registered users only

Download (598Kb) | Contact
[img] Text
227_2017_3281_MOESM3_ESM.pdf - Supplemental Material
Restricted to Registered users only

Download (487Kb) | Contact

Supplementary data:

Abstract

Marine copepods provide the major food-web link between primary producers and higher trophic levels, and their feeding ecology is of acute interest in light of global change impacts on food-web functioning. Recently, quantitative polymerase chain reaction (qPCR) protocols have been developed, which can complement classic diet quantification methods, such as stable isotope or fatty acid analyses tools. Here, we present first results of feeding experiments assessing sex- and stage-specific food intake by the ubiquitous calanoid copepod Acartia tonsa by 18S targeted qPCR and microscopic grazing assessment. In triplicated mixed-diet feeding treatments, three suitable A. tonsa diets, the cryptophyte Rhodomonas balthica, the haptophyte Isochrysis galbana, and the diatom Thalassiosira weissflogii, were offered in equal biomass proportions under constant conditions. Prey uptake substantially varied between different algal species, as did the extent of sex- and stage-specificity of prey uptake. Male adult copepods had higher R. balthica gut contents than females, and nauplii contained more of this prey source than copepodites or adult copepods in mixed treatments. A trend towards higher amounts of ingested T. weissflogii in adult females than in males and in nauplii than in other stages was detected. Genetic gut content quantifications indicated low feeding on I. galbana, and no consistent sex- or stage-specific differences of I. galbana content in A. tonsa. Our results highlight diet-specific feeding differences between Acartia life stages and sexes, which can have implications on food-web dynamics and specific nutrient transfer to higher trophic levels in copepod populations of varying age composition under changing environmental parameters, such as rising temperatures and increasing ocean acidification.

Document Type: Article
Research affiliation: OceanRep > GEOMAR > FB3 Marine Ecology > FB3-EOE-N Experimental Ecology - Food Webs
Refereed: Yes
DOI etc.: 10.1007/s00227-017-3281-z
ISSN: 0025-3162
Date Deposited: 12 Jan 2018 12:37
Last Modified: 12 Jan 2018 12:37
URI: http://eprints.uni-kiel.de/id/eprint/41437

Actions (login required)

View Item View Item

Document Downloads

More statistics for this item...