Biodiversity of green algae covering artificial hard substrate surfaces in a suburban environment: a case study using molecular approaches

Hallmann, Christine, Hoppert, Michael, Mudimu, Opayi, Friedl, Thomas and Kroth, P. (2016) Biodiversity of green algae covering artificial hard substrate surfaces in a suburban environment: a case study using molecular approaches Journal of Phycology, 52 (5). pp. 732-744. DOI 10.1111/jpy.12437.

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Abstract

In Middle European suburban environments green algae often cover open surfaces of artificial hard substrates. Microscopy reveals the Apatococcus/Desmococcus morphotype predominant over smaller coccoid forms. Adverse conditions as limited water availability connected with high PAR and UV irradiance may narrow the algal diversity to a few specialists in these subaerial habitats. We used rRNA gene cloning/sequencing from both, DNA extracts of the biofilms without culturing as well as cultures, for the unambiguous determination of the algal composition and to assess the algal diversity more comprehensively. The culture independent approach revealed in fact mainly just two genera (Apatococcus, Trebouxia) for all study sites and five molecular operational taxonomic units (OTUs) for a particular study site which based on microscopic observation was the one with the highest morphological diversity. The culture approach, however, revealed seven additional OTUs from five genera (Chloroidium, Coccomyxa, Coenochloris, Pabia, Klebsormidium) and an unidentified trebouxiophyte lineage for that same site; only two OTUs were shared by both approaches. Two OTUs or species were recovered for which references have been isolated only from Antarctica so far. However, the ITS sequence differences among them supported they are representing distinct populations of the same species. Within Apatococcus five clearly distinct groups of ITS sequences, each putatively representing a distinct species, were recovered with three or four such ITS types co-occurring at the same study site. Except for the streptophyte Klebsormidium only members of Trebouxiophyceae were detected suggesting these algae may be particularly well adapted to subaerial habitats. This article is protected by copyright. All rights reserved.

Biodiversity of green algae covering artificial hard substrate surfaces in a... | Request PDF. Available from: https://www.researchgate.net/publication/303914544_Biodiversity_of_green_algae_covering_artificial_hard_substrate_surfaces_in_a_suburban_environment_A_case_study_using_molecular_approaches [accessed Jan 10 2018].

Document Type: Article
Research affiliation: Kiel University > Kiel Marine Science
OceanRep > The Future Ocean - Cluster of Excellence
Refereed: Yes
DOI etc.: 10.1111/jpy.12437
ISSN: 00223646
Date Deposited: 10 Jan 2018 14:48
Last Modified: 01 Mar 2018 15:27
URI: http://eprints.uni-kiel.de/id/eprint/41397

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