Meprin Metalloproteases Generate Biologically Active Soluble Interleukin-6 Receptor to Induce Trans-Signaling

Arnold, Philipp, Boll, Inga, Rothaug, Michelle, Schumacher, Neele, Schmidt, Frederike, Wichert, Rielana, Schneppenheim, Janna, Lokau, Juliane, Pickhinke, Ute, Koudelka, Tomas, Tholey, Andreas, Rabe, Björn, Scheller, Jürgen, Lucius, Ralph, Garbers, Christoph, Rose-John, Stefan and Becker-Pauly, Christoph (2017) Meprin Metalloproteases Generate Biologically Active Soluble Interleukin-6 Receptor to Induce Trans-Signaling Scientific Reports, 7 . p. 44053. DOI 10.1038/srep44053.

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Supplementary data:

Abstract

Soluble Interleukin-6 receptor (sIL-6R) mediated trans-signaling is an important pro-inflammatory stimulus associated with pathological conditions, such as arthritis, neurodegeneration and inflammatory bowel disease. The sIL-6R is generated proteolytically from its membrane bound form and A Disintegrin And Metalloprotease (ADAM) 10 and 17 were shown to perform ectodomain shedding of the receptor in vitro and in vivo. However, under certain conditions not all sIL-6R could be assigned to ADAM10/17 activity. Here, we demonstrate that the IL-6R is a shedding substrate of soluble meprin α and membrane bound meprin β, resulting in bioactive sIL-6R that is capable of inducing IL-6 trans-signaling. We determined cleavage within the N-terminal part of the IL-6R stalk region, distinct from the cleavage site reported for ADAM10/17. Interestingly, meprin β can be shed from the cell surface by ADAM10/17 and the observation that soluble meprin β is not capable of shedding the IL-6R suggests a regulatory mechanism towards trans-signaling. Additionally, we observed a significant negative correlation of meprin β expression and IL-6R levels on human granulocytes, providing evidence for in vivo function of this proteolytic interaction.

Document Type: Article
Research affiliation: Kiel University > Kiel Marine Science
OceanRep > The Future Ocean - Cluster of Excellence
Refereed: Yes
DOI etc.: 10.1038/srep44053
ISSN: 2045-2322
Date Deposited: 08 Jan 2018 11:13
Last Modified: 01 Feb 2018 12:04
URI: http://eprints.uni-kiel.de/id/eprint/41255

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