Drosophila Fecal Sampling

Fink , Christine, von Frieling, Jakob, Knop, Mirjam and Roeder, Thomas (2017) Drosophila Fecal Sampling BIO-PROTOCOL, 7 (18). DOI 10.21769/BioProtoc.2547.

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Supplementary data:

Abstract

Fecal sampling is a non-invasive method which raises the possibility to study the development and the changes in the microbial community throughout different time points of a fly population or throughout different treatments. This method allows precise manipulation to trigger the fly’s physiology by nutritional interventions, bacterial infections or other stressors.

As in most other animals, the intestinal microbiota is essential for a healthy fly-life. Because Drosophila only harbors a relative simple bacterial community with a small variety of round about 8 to 10 different species, it is rather easy to build up the microbial community and to investigate microbial changes after treatment.

Another positive effect using the fly’s feces is that bacteria that are not part of the intestinal microbiome, for example Wolbachia, can be excluded directly from the analysis because they are not excreted.

Using this method, the generated datasets may reflect a good paradigm to study microbiome associated diseases in a simple fly model or furthermore, to test drugs in a high-throughput approach.

Document Type: Article
Keywords: Drosophila, Intestine, Microbiome, Fecal sampling, DNA isolation
Research affiliation: Kiel University > Kiel Marine Science
Kiel University
OceanRep > The Future Ocean - Cluster of Excellence
Refereed: Yes
DOI etc.: 10.21769/BioProtoc.2547
ISSN: 2331-8325
Date Deposited: 19 Dec 2017 16:16
Last Modified: 19 Dec 2017 16:16
URI: http://eprints.uni-kiel.de/id/eprint/40962

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