Down-Regulation of ZmEXPB6 (Zea maysβ-Expansin 6) Protein Is Correlated with Salt-mediated Growth Reduction in the Leaves ofZ. maysL.

Geilfus, Christoph-Martin, Ober, Dietrich, Eichacker, Lutz A., Mühling, Karl Hermann and Zörb, Christian (2015) Down-Regulation of ZmEXPB6 (Zea maysβ-Expansin 6) Protein Is Correlated with Salt-mediated Growth Reduction in the Leaves ofZ. maysL. Journal of Biological Chemistry, 290 (18). pp. 11235-11245. DOI 10.1074/jbc.M114.619718.

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Abstract

The salt-sensitive crop Zea mays L. shows a rapid leaf growth reduction upon NaCl stress. There is increasing evidence that salinity impairs the ability of the cell walls to expand, ultimately inhibiting growth. Wall-loosening is a prerequisite for cell wall expansion, a process that is under the control of cell wall-located expansin proteins. In this study the abundance of those proteins was analyzed against salt stress using gel-based two-dimensional proteomics and two-dimensional Western blotting. Results show that ZmEXPB6 (Z. mays β-expansin 6) protein is lacking in growth-inhibited leaves of salt-stressed maize. Of note, the exogenous application of heterologously expressed and metal-chelate-affinity chromatography-purified ZmEXPB6 on growth-reduced leaves that lack native ZmEXPB6 under NaCl stress partially restored leaf growth. In vitro assays on frozen-thawed leaf sections revealed that recombinant ZmEXPB6 acts on the capacity of the walls to extend. Our results identify expansins as a factor that partially restores leaf growth of maize in saline environments.

Document Type: Article
Refereed: Yes
DOI etc.: 10.1074/jbc.M114.619718
ISSN: 0021-9258
Date Deposited: 27 Jun 2017 13:01
Last Modified: 27 Jun 2017 13:01
URI: http://eprints.uni-kiel.de/id/eprint/38620

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