Construction and Screening of Marine Metagenomic Large Insert Libraries

Weiland-Bräuer, Nancy, Langfeldt, Daniela and Schmitz-Streit, Ruth (2017) Construction and Screening of Marine Metagenomic Large Insert Libraries Metagenomics. Methods in Molecular Biology. Humana Press, New York, pp. 23-42. DOI 10.1007/978-1-4939-6691-2_3.

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Abstract

The marine environment covers more than 70 % of the world's surface. Marine microbial communities are highly diverse and have evolved during extended evolutionary processes of physiological adaptations under the influence of a variety of ecological conditions and selection pressures. They harbor an enormous diversity of microbes with still unknown and probably new physiological characteristics. In the past, marine microbes, mostly bacteria of microbial consortia attached to marine tissues of multicellular organisms, have proven to be a rich source of highly potent bioactive compounds, which represent a considerable number of drug candidates. However, to date, the biodiversity of marine microbes and the versatility of their bioactive compounds and metabolites have not been fully explored. This chapter describes sampling in the marine environment, construction of metagenomic large insert libraries from marine habitats, and exemplarily one function based screen of metagenomic clones for identification of quorum quenching activities.

Document Type: Book chapter
Research affiliation: Kiel University
Kiel University > Kiel Marine Science
OceanRep > The Future Ocean - Cluster of Excellence > FO-R04
OceanRep > The Future Ocean - Cluster of Excellence
Refereed: Yes
DOI etc.: 10.1007/978-1-4939-6691-2_3
ISSN: 1064-3745
Projects: Future Ocean
Date Deposited: 13 Mar 2017 13:33
Last Modified: 05 Feb 2018 11:55
URI: http://eprints.uni-kiel.de/id/eprint/36953

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