Enhancing metaproteomics The value of models and defined environmental microbial systems

Herbst, F. A., Lunsmann, V., Kjeldal, H., Jehmlich, N., Tholey, Andreas, von Bergen, M., Nielsen, J. L., Hettich, R. L., Seifert, J. and Nielsen, P. H. (2016) Enhancing metaproteomics The value of models and defined environmental microbial systems Proteomics, 16 (5). pp. 783-798. DOI 10.1002/pmic.201500305.

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Supplementary data:


Metaproteomicsthe large-scale characterization of the entire protein complement of environmental microbiota at a given point in timehas provided new features to study complex microbial communities in order to unravel these black boxes. New technical challenges arose that were not an issue for classical proteome analytics before that could be tackled by the application of different model systems. Here, we review different current and future model systems for metaproteome analysis. Following a short introduction to microbial communities and metaproteomics, we introduce model systems for clinical and biotechnological research questions including acid mine drainage, anaerobic digesters, and activated sludge. Model systems are useful to evaluate the challenges encountered within (but not limited to) metaproteomics, including species complexity and coverage, biomass availability, or reliable protein extraction. The implementation of model systems can be considered as a step forward to better understand microbial community responses and ecological functions of single member organisms. In the future, improvements are necessary to fully explore complex environmental systems by metaproteomics.

Document Type: Article
Additional Information: Times Cited: 3 Herbst, Florian-Alexander Luensmann, Vanessa Kjeldal, Henrik Jehmlich, Nico Tholey, Andreas von Bergen, Martin Nielsen, Jeppe Lund Hettich, Robert L. Seifert, Jana Nielsen, Per Halkjaer Si
Research affiliation: Kiel University > Kiel Marine Science
OceanRep > The Future Ocean - Cluster of Excellence
Refereed: Yes
DOI etc.: 10.1002/pmic.201500305
ISSN: 1615-9853
Projects: Future Ocean
Date Deposited: 27 Feb 2017 09:23
Last Modified: 01 Feb 2018 12:08
URI: http://eprints.uni-kiel.de/id/eprint/36129

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