Fractionation of Plant Bioactives from Black Carrots (Daucus carota subspecies sativus varietas atrorubens Alef.) by Adsorptive Membrane Chromatography and Analysis of Their Potential Anti-Diabetic Activity

Esatbeyoglu, T., Rodriguez-Werner, M., Schlosser, A., Liehr, M., Ipharraguerre, I., Winterhalter, P. and Rimbach, Gerald (2016) Fractionation of Plant Bioactives from Black Carrots (Daucus carota subspecies sativus varietas atrorubens Alef.) by Adsorptive Membrane Chromatography and Analysis of Their Potential Anti-Diabetic Activity Journal of Agricultural and Food Chemistry, 64 (29). pp. 5901-5908. DOI 10.1021/acs.jafc.6b02292.

Full text not available from this repository.

Supplementary data:

Abstract

Black and purple carrots have attracted interest as colored extracts for coloring food due to their high content of anthocyanins. This study aimed to investigate the polyphenol composition of black carrots. Particularly, the identification and quantification of phenolic compounds of the variety Deep Purple carrot (DPC), which presents a very dark color, was performed by HPLC-PDA and HPLC-ESI-MS" analyses. The separation of polyphenols from a DPC XAD-7 extract into an anthocyanin fraction (AF) and co-pigment fraction (CF; primarily phenolic acids) was carried out by membrane chromatography. Furthermore, possible anti-diabetic effects of the DPC XAD-7 extract and its AF and CF were determined. DPC samples (XAD-7, CF, and AF) inhibited alpha-amylase and alpha-glucosidase in a dose-dependent manner. Moreover, DPC XAD-7 and chlorogenic acid, but not DPC CF and DPC AF, caused a moderate inhibition of intestinal glucose uptake in Caco-2 cells. However, DPC samples did not affect glucagon-like peptide-1 (GLP-1) secretion and dipeptidyl peptidase IV (DPP-4) activity. Overall, DPC exhibits an inhibitory effect on alpha-amylase and alpha-glucosidase activity and on cellular glucose uptake indicating potential anti-diabetic properties.

Document Type: Article
Additional Information: Times Cited: 0 Esatbeyoglu, Tuba Rodriguez-Werner, Miriam Schloesser, Anke Liehr, Martin Ipharraguerre, Ignacio Winterhalter, Peter Rimbach, Gerald
Research affiliation: Kiel University
OceanRep > The Future Ocean - Cluster of Excellence
Refereed: Yes
DOI etc.: 10.1021/acs.jafc.6b02292
ISSN: 0021-8561
Projects: Future Ocean
Date Deposited: 01 Mar 2017 08:22
Last Modified: 01 Mar 2017 08:22
URI: http://eprints.uni-kiel.de/id/eprint/36081

Actions (login required)

View Item View Item