Chemical Characterization, Free Radical Scavenging, and Cellular Antioxidant and Anti-Inflammatory Properties of a Stilbenoid-Rich Root Extract of Vitis vinifera

Esatbeyoglu, T., Ewald, P., Yasui, Y., Yokokawa, H., Wagner, A. E., Matsugo, S., Winterhalter, P. and Rimbach, Gerald (2016) Chemical Characterization, Free Radical Scavenging, and Cellular Antioxidant and Anti-Inflammatory Properties of a Stilbenoid-Rich Root Extract of Vitis vinifera Oxidative Medicine and Cellular Longevity . DOI 10.1155/2016/8591286.

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Abstract

Dietary stilbenoids are receiving increasing attention due to their potential health benefits. However, most studies concerning the bioactivity of stilbenoids were conducted with pure compounds, for example, resveratrol. The aim of this study was to characterize a complex root extract of Vitis vinifera in terms of its free radical scavenging and cellular antioxidant and anti-inflammatory properties. HPLC-ESI-MS/MS analyses of the root extract of Vitis vinifera identified seven stilbenoids including two monomeric (resveratrol and piceatannol), two dimeric (trans-epsilon-viniferin and ampelopsin A), one trimeric (miyabenol C), and two tetrameric (r-2-viniferin = vitisin A and r-viniferin = vitisin B) compounds whichmay mediate its biological activity. Electron spin resonance and spin trapping experiments indicate that the root extract scavenged 2,2-diphenyl-1-picrylhydrazyl, hydroxyl, galvinoxyl, and superoxide free radicals. On a cellular level it was observed that the root extract of Vitis vinifera protects against hydrogen peroxideinduced-DNA-damage and inducesNrf2 and its target genes heme oxygenase-1 and gamma-glutamylcysteine synthetase. Furthermore, the root extract could induce the antiatherogenic hepatic enzyme paraoxonase 1 and downregulate proinflammatory gene expression (interleukin 1 beta, inducible nitric oxide synthase) in macrophages. Collectively our data suggest that the root extract of Vitis vinifera exhibits free radical scavenging as well as cellular antioxidant and anti-inflammatory properties.

Document Type: Article
Additional Information: Times Cited: 0 Esatbeyoglu, Tuba Ewald, Philipp Yasui, Yoshiaki Yokokawa, Haruka Wagner, Anika E. Matsugo, Seiichi Winterhalter, Peter Rimbach, Gerald
Research affiliation: Kiel University
OceanRep > The Future Ocean - Cluster of Excellence
Refereed: Yes
DOI etc.: 10.1155/2016/8591286
ISSN: 1942-0900
Projects: Future Ocean
Date Deposited: 25 Feb 2017 07:04
Last Modified: 25 Feb 2017 07:04
URI: http://eprints.uni-kiel.de/id/eprint/36080

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