Williamsia herbipolensis sp. nov., isolated from the phyllosphere of Arabidopsis thaliana

Kämpfer, Peter, Busse, Hans-Jürgen, Horn, Hannes, Abdelmohsen, Usama R., Hentschel, Ute and Glaeser, Stefanie (2016) Williamsia herbipolensis sp. nov., isolated from the phyllosphere of Arabidopsis thaliana International Journal of Systematic and Evolutionary Microbiology, 66 (11). pp. 4609-4613. DOI 10.1099/ijsem.0.001398.

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Supplementary data:

Abstract

A Gram-stain-positive, non-endospore-forming actinobacterium (ARP1T) was isolated from the phyllosphere of Arabidopsis thaliana. On the basis of 16S rRNA gene sequence phylogeny strain ARP1T was placed into the genus Williamsia and the closest related species were Williamsia phyllosphaerae (98.5 % 16S rRNA gene sequence similarity), Williamsia deligens (98.5 %), Williamsia maris (98.3 %) and Williamsia serinedens (98.2 %). Genome-based comparison indicated a clear distinction to the type strains of those species with pairwise average nucleotide identities (ANI) between 76.4–78.4 %. The quinone system of strain ARP1T consisted predominantly of menaquinones MK-9(H2), MK-7(H2) and MK-8(H2), and the polar lipid profile contained the major compound diphosphatidylglycerol, and moderate amounts of phosphatidylethanolamine, phosphatidylglycerol and numerous unidentified lipids. Mycolic acids were present. These chemotaxonomic traits and the major fatty acids, which were C16 : 1ω7c, C16 : 0, C18 : 0, C18 : 1ω9c and tuberculostearic acid supported the affiliation of strain ARP1T to the genus Williamsia . Genotypic, physiological and biochemical testing revealed clear differences of strain ARP1T to the most closely related species of the genus Williamsia . Therefore strain ARP1T represents a novel species of this genus, for which the name Williamsia herbipolensis sp. nov. is proposed. The type strain is ARP1T (=DSM 46872T=LMG 28679T).

Document Type: Article
Additional Information: The GenBank/EMBL/DDBJ accession number of the 16S rRNA gene sequences of strain ARP1T is KP676047. The Whole Genome Shotgun (WGS) project number of strain ARP1T in Genbank is BioProject PRJNA272726 with accession number JXYP00000000. The locus tag of the 16S rRNA gene sequence is Ga0077739_144.
Keywords: New species, Williamsia, Williamsia herbipolensis sp. nov.
Research affiliation: OceanRep > GEOMAR > FB3 Marine Ecology > FB3-MI Marine Microbiology
Refereed: Yes
DOI etc.: 10.1099/ijsem.0.001398
ISSN: 1466-5026
Related URLs:
Date Deposited: 19 Dec 2016 11:22
Last Modified: 02 May 2017 13:45
URI: http://eprints.uni-kiel.de/id/eprint/35300

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