De Novo Assembly and Genome Analyses of the Marine-Derived Scopulariopsis brevicaulis Strain LF580 Unravels Life-Style Traits and Anticancerous Scopularide Biosynthetic Gene Cluster

Kumar, Abhishek, Henrissat, Bernard, Arvas, Mikko, Syed, Muhammad Fahad, Thieme, Nils, Benz, J. Philipp, Sorensen, Jens Laurids, Record, Eric, Poeggeler, Stefanie and Kempken, Frank (2015) De Novo Assembly and Genome Analyses of the Marine-Derived Scopulariopsis brevicaulis Strain LF580 Unravels Life-Style Traits and Anticancerous Scopularide Biosynthetic Gene Cluster Plos One, 10 (10).

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Abstract

The marine-derived Scopulariopsis brevicaulis strain LF580 produces scopularides A and B, which have anticancerous properties. We carried out genome sequencing using three next-generation DNA sequencing methods. De novo hybrid assembly yielded 621 scaffolds with a total size of 32.2 Mb and 16298 putative gene models. We identified a large non-ribosomal peptide synthetase gene (nrps1) and supporting pks2 gene in the same biosynthetic gene cluster. This cluster and the genes within the cluster are functionally active as confirmed by RNA-Seq. Characterization of carbohydrate-active enzymes and major facilitator superfamily (MFS)-type transporters lead to postulate S. brevicaulis originated from a soil fungus, which came into contact with the marine sponge Tethya aurantium. This marine sponge seems to provide shelter to this fungus and micro-environment suitable for its survival in the ocean. This study also builds the platform for further investigations of the role of life-style and secondary metabolites from S. brevicaulis.

Document Type: Article
Additional Information: Times Cited: 1
Research affiliation: OceanRep > The Future Ocean - Cluster of Excellence
Kiel University
OceanRep > The Future Ocean - Cluster of Excellence > FO-R03
Refereed: Yes
ISSN: 1932-6203
Projects: Future Ocean
Date Deposited: 18 Oct 2016 03:45
Last Modified: 19 Dec 2017 12:45
URI: http://eprints.uni-kiel.de/id/eprint/32562

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