Investigation on the production of secondary metabolites from anoxygenic phototrophic bacteria

Sun, Min (2016) Investigation on the production of secondary metabolites from anoxygenic phototrophic bacteria (Doctoral thesis/PhD), Christian-Albrechts-Universität Kiel, Kiel, Germany, 160 pp

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Abstract

Secondary metabolites production of representative anoxygenic phototrophic bacteria were studied by culture-dependent analyses, PCR-based screening of selected strains and genome-based search using available sequence information of 62 phototrophic bacteria from public databases. Metabolite profiles of Allochromatium vinosum strain MT86 revealed a terpene like new compound produced under different growth conditions. Bioactivity tests showed some inhibitory activity of this substance against B. subtilis and S. lentus. Genome-based analysis of genes for secondary metabolite biosynthesis revealed that terpene biosynthesis gene clusters occupied the most significant fraction from 32 complete genomes of anoxygenic phototrophic bacteria. In a PCR-based screening with specific primers, genes of type I pks, type II pks and nrps could not be identified within ten phototrophic bacteria, while the presence of phzE could be approved in eight of them, including Ach. vinosum strain MT86.

Document Type: Thesis (Doctoral thesis/PhD)
Thesis Advisors: Imhoff, Johannes F. and Hentschel, Ute
Keywords: secondary metabolite production, anoxygenic phototrophic bacteria, Allochromatium vinosum, antiSMASH, PKS, NRPS, phenazine
Research affiliation: OceanRep > GEOMAR > FB3 Marine Ecology > FB3-MI Marine Microbiology
Date Deposited: 26 Feb 2016 09:15
Last Modified: 26 Feb 2016 09:15
URI: http://eprints.uni-kiel.de/id/eprint/31563

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