Streptocollin, a Type IV Lanthipeptide Produced by Streptomyces collinus Tü 365

Iftime, Dumitrita, Jasyk, Martin, Kulik, Andreas, Imhoff, Johannes F., Stegmann, Evi, Wohlleben, Wolfgang, Süssmuth, Roderich D. and Weber, Tilmann (2015) Streptocollin, a Type IV Lanthipeptide Produced by Streptomyces collinus Tü 365 ChemBioChem, 16 (18). pp. 2615-2623. DOI 10.1002/cbic.201500377.

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Abstract

Lanthipeptides are ribosomally synthesized and post-translationally modified microbial secondary metabolites. Here, we report the identification and isolation of streptocollin from Streptomyces collinus Tü 365, a new member of class IV lanthipeptides. Insertion of the constitutive ermE* promoter upstream of the lanthipeptide synthetase gene stcL resulted in peptide production. The streptocollin gene cluster was heterologously expressed in S. coelicolor M1146 and M1152 with 3.5- and 5.5-fold increased yields, respectively. The structure and ring topology of streptocollin were determined by high resolution MS/MS analysis. Streptocollin contains four macrocyclic rings, with one lanthionine and three methyllanthionine residues. To the best of our knowledge, this is the first report on the isolation of a class IV lanthipeptide in preparative amounts, and on the successful heterologous expression of a class IV lanthipeptide gene cluster.

Document Type: Article
Additional Information: Supplementary information: cbic_201500377_sm_miscellaneous_information.pdf The document includes (i) Media and culture conditions; (ii) General protocols; (iii) Figure S1 Cosmid library screening; (iv) Table S1 Strains and plasmids used in this study; (v) Table S2 ppm values of streptocollin fragments; (vi) Figure S2 Full-size spectrum of streptocollin fragmentation; (vii) Figure S3 TIC-MS (total ion chromatogram) of streptocollin; (viii) Table S3 Growth conditions for indicator strains used in antimicrobial assays; (ix) References - WOS:000367720300012
Keywords: Streptomyces; genomics; lantibiotics; natural products; type IV lanthipeptide
Research affiliation: OceanRep > GEOMAR > FB3 Marine Ecology > FB3-MI Marine Microbiology
Refereed: Yes
DOI etc.: 10.1002/cbic.201500377
ISSN: 1439-4227
Projects: Future Ocean
Date Deposited: 18 Nov 2015 11:23
Last Modified: 19 Dec 2017 12:45
URI: http://eprints.uni-kiel.de/id/eprint/30276

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