Proteomic Analysis of Anti-Cancerous Scopularide Production by a Marine Microascus brevicaulis Strain and Its UV Mutant

Kramer, Annemarie, Beck, Hans Christian, Kumar, Abhishek, Kristensen, Lars Peter, Imhoff, Johannes F. and Labes, Antje (2015) Proteomic Analysis of Anti-Cancerous Scopularide Production by a Marine Microascus brevicaulis Strain and Its UV Mutant PLoS ONE, 10 (10). e0140047. DOI 10.1371/journal.pone.0140047.

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The marine fungus Microascus brevicaulis strain LF580 is a non-model secondary metabolite producer with high yields of the two secondary metabolites scopularides A and B, which exhibit distinct activities against tumour cell lines. A mutant strain was obtained using UV mutagenesis, showing faster growth and differences in pellet formation besides higher production levels. Here, we show the first proteome study of a marine fungus. Comparative proteomics were applied to gain deeper understanding of the regulation of production and of the physiology of the wild type strain and its mutant. For this purpose, an optimised protein extraction protocol was established. In total, 4759 proteins were identified. The central metabolic pathway of strain LF580 was mapped using the KEGG pathway analysis and GO annotation. Employing iTRAQ labelling, 318 proteins were shown to be significantly regulated in the mutant strain: 189 were down- and 129 upregulated. Proteomics are a powerful tool for the understanding of regulatory aspects: The differences on proteome level could be attributed to limited nutrient availability in the wild type strain due to a strong pellet formation. This information can be applied for optimisation on strain and process level. The linkage between nutrient limitation and pellet formation in the non-model fungus M. brevicaulis is in consensus with the knowledge on model organisms like Aspergillus niger and Penicillium chrysogenum

Document Type: Article
Additional Information: Data Availability Statement: All relevant data are available in the Supporting Information files or via the Pangaea database doi:
Keywords: Mutant strains; Proteomes; Protein extraction; Enzyme regulation; Fungi; Cell disruption; Protein metabolism; Biosynthesis
Research affiliation: OceanRep > The Future Ocean - Cluster of Excellence
OceanRep > GEOMAR > FB3 Marine Ecology > FB3-MN Marine natural products chemistry
OceanRep > GEOMAR > FB3 Marine Ecology > FB3-MI Marine Microbiology
Kiel University
Refereed: Yes
DOI etc.: 10.1371/journal.pone.0140047
ISSN: 1932-6203
Projects: KIWIZ, Future Ocean
Date Deposited: 18 Nov 2015 11:19
Last Modified: 19 Dec 2017 12:43

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