Genetic analysis of sea-ice bacterial communities of the Western Baltic Sea using an improved double gradient method

Petri, Ralf and Imhoff, Johannes F. (2001) Genetic analysis of sea-ice bacterial communities of the Western Baltic Sea using an improved double gradient method Polar Biology (4). pp. 252-257. DOI 10.1007/s003000000205.

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Abstract

The bacterial diversity of sea ice from Kiel Bight obtained during the rare event of solid ice cover in spring 1996 was analysed by molecular genetic approaches using an improved double gradient denaturing gradient gel electrophoretic method (DG-DGGE) to separate 16S rDNA fragments of approximately 500 bp. The excellent separation of individual bands within these gradient gels allowed us to obtain sequence information and to allocate the phylogenetic position of representative bacteria from the sea ice. The band pattern of the gradient gels revealed a vertical stratification of the bacterial species distribution within the ice and the presence of characteristic bacteria for each layer. According to their 16S rDNA sequences, major bands of the gradient gels represented bacteria closely related to fermenting species of the genera Propionibacterium and Bacteroides and to anoxygenic phototrophic purple sulfur bacteria (Chromatiaceae). Their abundance in horizons of the inner ice core may indicate the existence of oxygen-deficient and anoxic zones or niches and possible primary production by anoxygenic photosynthesis within the investigated Baltic Sea sea ice. This is the first phylogenetic evidence of the presence, and most probably the development, of phototrophic purple sulfur bacteria in sea ice.

Document Type: Article
Research affiliation: OceanRep > GEOMAR > FB3 Marine Ecology > FB3-MI Marine Microbiology
Refereed: Yes
DOI etc.: 10.1007/s003000000205
ISSN: 0722-4060
Date Deposited: 18 Feb 2008 17:25
Last Modified: 09 Nov 2016 14:35
URI: http://eprints.uni-kiel.de/id/eprint/2995

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