Influence of mathematical models and correction factors on binding results of polyphenols and retinol with beta-lactoglobulin measured with fluorescence quenching

Keppler, Julia Katharina, Stuhldreier, Mayra Christina, Temps, Friedrich and Schwarz, Karin (2014) Influence of mathematical models and correction factors on binding results of polyphenols and retinol with beta-lactoglobulin measured with fluorescence quenching Food Biophysics, 9 (2). pp. 158-168.

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Abstract

beta-Lactoglobulin is frequently researched as food grade nanotransporter for hydrophobic compounds like polyphenols and fatty soluble vitamins. The effects of self-fluorescence and inner filtering caused by ligands on the analysis of ligand-beta-lactoglobulin-binding were evaluated using fluorescence quenching data. Without correction for self-fluorescence or inner filtering the binding constants are either overestimated or binding is erroneously assumed. Additionally, the variance between the derived numbers of binding sites resulting by using different mathematical models with the same data set is about 50 %. Thus here we suggest the use of at least two very different mathematical approaches when deriving fluorescence titration data and evaluating the underlying restrictions of the respective model thoroughly. The greatest differences were found to concern K'a or K'd, whereas n seems to be relatively stable. The Cogan plot seems to be the best choice for FQ calculations, given that it does not require an estimation of added ligand concentration and that it is insensitive to residual fluorescence.

Document Type: Article
Additional Information: Times Cited: 0 0
Research affiliation: Kiel University
OceanRep > The Future Ocean - Cluster of Excellence
ISSN: 1557-1858
Projects: Future Ocean
Date Deposited: 30 Mar 2015 12:28
Last Modified: 30 Mar 2015 12:28
URI: http://eprints.uni-kiel.de/id/eprint/27744

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