Time and concentration dependency of MacroGard® induced apoptosis

Miest, Joanna J. and Hoole, D. (2015) Time and concentration dependency of MacroGard® induced apoptosis Fish & Shellfish Immunology, 42 (2). pp. 363-366. DOI 10.1016/j.fsi.2014.11.009.

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Supplementary data:


• β-glucan induces apoptosis in a time and concentration dependent manner.
• ≥500 μg/ml induce apoptosis in pronephric cells in vitro.
• Apoptosis is observed after 6 h of incubation.

In previous studies an effect of β-glucan on apoptosis in fish was noted and in this investigation we determine the time and concentration dependency of this effect. Primary cell cultures of pronephric carp cells were incubated for 6, 24, 48 h with various concentrations ranging from 0 - 1000 μg/ml of MacroGard® β-glucan. Apoptosis was monitored via acridine orange staining. Results indicate a clear effect of time and concentration on the induction of apoptosis in vitro, with only concentration ≥ 500 μg/ml causing significantly higher percentages of apoptotic cells. Apoptosis was detected after 6 h. This concentration dependent effect has to be considered when studying apoptosis in relation to immunostimulation.

Document Type: Article
Keywords: Apoptosis; beta-glucan; carp; concentration/time dependency
Research affiliation: OceanRep > GEOMAR > FB3 Marine Ecology > FB3-EV Evolutionary Ecology of Marine Fishes
Refereed: Yes
DOI etc.: 10.1016/j.fsi.2014.11.009
ISSN: 1050-4648
Projects: NEMO
Date Deposited: 04 Dec 2014 10:43
Last Modified: 05 Jan 2017 13:33
URI: http://eprints.uni-kiel.de/id/eprint/26202

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