Pathogenesis and inflammatory response to Edwardsiella tarda infection in the zebrafish

Pressley, Meagan E., Phelan, Peter E., Witten, P. Eckhard, Mellon, Mark T. and Kim, Carol H. (2005) Pathogenesis and inflammatory response to Edwardsiella tarda infection in the zebrafish Developmental and Comparative Immunology, 29 . pp. 501-513. DOI 10.1016/j.dci.2004.10.007.

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The zebrafish (Danio rerio) is a widely used model for developmental biology, neurobiology, toxicology, and genetic disease. Recently, the zebrafish has been recognized as a valuable model for infectious disease and immunity. In this study the pathogenesis and inflammatory cytokine response of zebrafish to experimental Edwardsiella tarda infection was characterized. In challenge experiments, zebrafish embryos were susceptible to infection by immersion. Adult fish were susceptible to challenge by intraperitoneal (ip) injection but not static immersion unless the epithelial layer was perturbed by scraping prior to exposure. To determine if E. tarda infection induces a typical acute inflammatory response, mRNA expression levels of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNFα) were assessed by quantitative real-time PCR. The expression levels of IL-1β and TNFα were significantly upregulated in infected zebrafish embryos and adults. The methods developed in this study will be particularly valuable for targeted gene disruption studies of host immune components and in zebrafish genetic screens.

Document Type: Article
Keywords: Zebrafish; Pathogenesis; Edwardsiella tarda; Experimental infection; Inflammatory response
Research affiliation: OceanRep > GEOMAR > FB3 Marine Ecology > FB3-EV Evolutionary Ecology of Marine Fishes
Refereed: Yes
DOI etc.: 10.1016/j.dci.2004.10.007
ISSN: 0145-305X
Date Deposited: 13 Feb 2009 10:56
Last Modified: 02 Nov 2016 13:54

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