Unraveling the Genetic Basis of Seed Tocopherol Content and Composition in Rapeseed (Brassica napus L.)

Wang, X. X., Zhang, C. Y., Li, L., Fritsche, S., Endrigkeit, J., Zhang, W. Y., Long, Y., Jung, Christian and Meng, J. L. (2012) Unraveling the Genetic Basis of Seed Tocopherol Content and Composition in Rapeseed (Brassica napus L.) PLoS ONE, 7 (11).

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Abstract

Background: Tocopherols are important antioxidants in vegetable oils; when present as vitamin E, tocopherols are an essential nutrient for humans and livestock. Rapeseed (Brassica napus L, AACC, 2 n = 38) is one of the most important oil crops and a major source of tocopherols. Although the tocopherol biosynthetic pathway has been well elucidated in the model photosynthetic organisms Arabidopsis thaliana and Synechocystis sp. PCC6803, knowledge about the genetic basis of tocopherol biosynthesis in seeds of rapeseed is scant. This project was carried out to dissect the genetic basis of seed tocopherol content and composition in rapeseed through quantitative trait loci (QTL) detection, genome-wide association analysis, and homologous gene mapping. Methodology/Principal Findings: We used a segregating Tapidor x Ningyou7 doubled haploid (TNDH) population, its reconstructed F-2 (RC-F-2) population, and a panel of 142 rapeseed accessions (association panel). Genetic effects mainly contributed to phenotypic variations in tocopherol content and composition; environmental effects were also identified. Thirty-three unique QTL were detected for tocopherol content and composition in TNDH and RC-F-2 populations. Of these, seven QTL co-localized with candidate sequences associated with tocopherol biosynthesis through in silico and linkage mapping. Several near-isogenic lines carrying introgressions from the parent with higher tocopherol content showed highly increased tocopherol content compared with the recurrent parent. Genome-wide association analysis was performed with 142 B. napus accessions. Sixty-one loci were significantly associated with tocopherol content and composition, 11 of which were localized within the confidence intervals of tocopherol QTL. Conclusions/Significance: This joint QTL, candidate gene, and association mapping study sheds light on the genetic basis of seed tocopherol biosynthesis in rapeseed. The sequences presented here may be used for marker-assisted selection of oilseed rape lines with superior tocopherol content and composition.

Document Type: Article
Additional Information: Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan, Peoples R China. Univ Kiel, Plant Breeding Inst, Kiel, Germany. Yangtze Univ, Sch Agr, Inst Plant Genet & Breeding, Jinzhou, Peoples R China. Meng, JL (reprint author), Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan, Peoples R China. jmeng@mail.hzau.edu.cn
Keywords: quantitative trait loci vitamin-e content genome-wide association oilseed rape arabidopsis-thaliana linkage disequilibrium population-structure 4-hydroxyphenylpyruvate dioxygenase e biosynthesis plants
Research affiliation: Kiel University > Kiel Marine Science
OceanRep > The Future Ocean - Cluster of Excellence
Kiel University
Refereed: Yes
ISSN: 1932-6203
Projects: Future Ocean
Date Deposited: 14 May 2014 09:07
Last Modified: 01 Aug 2018 09:30
URI: http://eprints.uni-kiel.de/id/eprint/24294

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