Expression of human carbonyl reductase 3 (CBR3; SDR21C2) is inducible by pro-inflammatory stimuli

Malatkova, P., Ebert, B., Wsol, V. and Maser, Edmund (2012) Expression of human carbonyl reductase 3 (CBR3; SDR21C2) is inducible by pro-inflammatory stimuli Biochemical and Biophysical Research Communications, 420 (2). pp. 368-373.

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Abstract

Until today, the physiologic role of human carbonyl reductase 3 (CBR3; SDR21C2), a member of the short-chain dehydrogenase/reductase superfamily remains obscure. Since the transcriptional regulation is closely related to the function of a protein, elucidation of the regulation of CBR3 should help to understand its physiologic role. We recently identified CBR3 as a novel target gene of Nrf2, a cellular sensor of oxidative stress. In this study, we provide for the first time evidence that pro-inflammatory stimuli induce the expression of the CBR3 gene. Treatment of human cancer cells HT-29 (colon) and HepG2 (liver) with TNF-alpha, IL-1 beta, and LPS induced CBR3 expression differentially. While TNF-alpha. (50 ng/ml) or IL-1 beta (1 and 10 ng/ml), induced CBR3 mRNA expression in HT-29 cells (up to 10-fold) and HepG2 cells (up to 20-fold), LPS activated the CBR3 gene only in HepG2 cells. Furthermore, overexpression of the NF kappa B subunits p65 and p50 alone or in combination elevated CBR3 mRNA levels (3.9-fold) in HT-29 cells. According to our results. CBR3 is a novel target gene of inflammatory stimuli, and elucidation of its detailed role in inflammation deserves further investigation. (C) 2012 Elsevier Inc. All rights reserved.

Document Type: Article
Additional Information: Univ Med Sch Schleswig Holstein, Inst Toxicol & Pharmacol Nat Scientists, D-24105 Kiel, Germany. Charles Univ Prague, Fac Pharm, Dept Biochem Sci, CZ-50005 Hradec Kralove, Czech Republic. Maser, E (reprint author), Univ Med Sch Schleswig Holstein, Inst Toxicol & Pharmacol Nat Scientists, Brunswiker Str 10, D-24105 Kiel, Germany. maser@toxi.uni-kiel.de
Keywords: Carbonyl reductase 3 Inflammation NF kappa B Transcriptional regulation nf-kappa-b transcription factors promoter binding target alpha cells nrf2
Research affiliation: OceanRep > The Future Ocean - Cluster of Excellence
Kiel University
ISSN: 0006-291X
Projects: Future Ocean
Date Deposited: 14 May 2014 09:49
Last Modified: 14 May 2014 09:49
URI: http://eprints.uni-kiel.de/id/eprint/24110

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