E-GEOD-28870.processed.1.zip

Fehsenfeld, Sandra, Kiko, Rainer, Appelhans, Yasmin, Towle, D. W., Zimmer, Martin and Melzner, Frank (2012) E-GEOD-28870.processed.1.zip ArrayExpress Archive .

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Abstract

In order to promote our understanding of the responses of green crab acid-base regulatory epithelia to high pCO2, Baltic Sea green crabs were exposed to a pCO2 of 400 Pa for 3 and 7 days after which posterior gills 7 and 9 were sampled. Gills were then subsequently screened for differentially expressed gene transcripts using a 4,462-feature microarray developed by Towle et al. 2010. For each experimental block (gill7-day3, gill7-day7, gill9-day3, gill9-day7), 6 replicate samples were obtained for control (= 39 Pa) and elevated (= 400 Pa) pCO2 exposed animals. Each microarray slide included 4 technical replicates for each transcript and was hybridized with one control pCO2 (labelled with AlexaFluor555) and one elevated pCO2 cDNA (labelled with AlexaFluor647). Lowess-normalized gene expression was calculated as the log2 of the ratio of the fluorescence intensity of the CO2-treatment cDNA to the fluorescence intensity of the control cDNA (log2 ratio=F635/F532).

Document Type: Article
Research affiliation: Kiel University > Faculty of Mathematics and Natural Sciences > Institute of Zoology and Zoological Museum
OceanRep > The Future Ocean - Cluster of Excellence
OceanRep > GEOMAR > FB3 Marine Ecology > FB3-EOE-B Experimental Ecology - Benthic Ecology
Projects: Future Ocean
Date Deposited: 14 May 2014 10:12
Last Modified: 14 May 2014 10:12
URI: http://eprints.uni-kiel.de/id/eprint/23916

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