Evolution of MHC class I genes in the endangered loggerhead sea turtle (Caretta caretta) revealed by 454 amplicon sequencing

Stiebens, Victor, Merino, S. E., Chain, F. J. J. and Eizaguirre, Christophe (2013) Evolution of MHC class I genes in the endangered loggerhead sea turtle (Caretta caretta) revealed by 454 amplicon sequencing BMC Evolutionary Biology, 13 . No. 95. DOI 10.1186/1471-2148-13-95.

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Abstract

Background: In evolutionary and conservation biology, parasitism is often highlighted as a major selective pressure. To fight against parasites and pathogens, genetic diversity of the immune genes of the major histocompatibility complex (MHC) are particularly important. However, the extensive degree of polymorphism observed in these genes makes it difficult to conduct thorough population screenings.

Methods: We utilized a genotyping protocol that uses 454 amplicon sequencing to characterize the MHC class I in the endangered loggerhead sea turtle (Caretta caretta) and to investigate their evolution at multiple relevant levels of organization.

Results: MHC class I genes revealed signatures of trans-species polymorphism across several reptile species. In the studied loggerhead turtle individuals, it results in the maintenance of two ancient allelic lineages. We also found that individuals carrying an intermediate number of MHC class I alleles are larger than those with either a low or high number of alleles.

Conclusions: Multiple modes of evolution seem to maintain MHC diversity in the loggerhead turtles, with relatively high polymorphism for an endangered species.

Document Type: Article
Additional Information: WOS:000318937700001
Keywords: Major histocompatibility complex; Loggerhead sea turtle; Trans-species polymorphism; Reptiles; Intermediate diversity; MAJOR HISTOCOMPATIBILITY COMPLEX; TUATARA SPHENODON-SPP.; AMINO-ACID SITES; 3-SPINED STICKLEBACK; PARASITE SELECTION; MATE CHOICE; CAPE-VERDE; POLYMORPHISM; DIVERSITY; LOCI
Research affiliation: OceanRep > GEOMAR > FB3 Marine Ecology > FB3-EV Evolutionary Ecology of Marine Fishes
Refereed: Yes
DOI etc.: 10.1186/1471-2148-13-95
ISSN: 1471-2148
Date Deposited: 29 Apr 2013 07:17
Last Modified: 20 Jun 2016 12:04
URI: http://eprints.uni-kiel.de/id/eprint/21178

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