Emended description of the genus Rhodothalassium Imhoff et al., 1998 and proposal of Rhodothalassiaceae fam. nov. and Rhodothalassiales ord. nov

Ramana, V. Venkata , Chakravarthy, S. Kalyana, Ramaprasad, E. V. V., Thiel, Vera, Imhoff, Johannes F., Sasikala, Ch. and Ramana, Ch. V. (2013) Emended description of the genus Rhodothalassium Imhoff et al., 1998 and proposal of Rhodothalassiaceae fam. nov. and Rhodothalassiales ord. nov Systematic and Applied Microbiology, 36 (1). pp. 28-32. DOI 10.1016/j.syapm.2012.09.003.

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Abstract

Five strains (JA325, JA389, JA473, JA563 and JA582) of Gram stain-negative, vibrioid to spiral shaped, phototrophic purple bacteria were isolated from solar salterns of India. All strains contained bacteriochlorophyll-a and carotenoids of the spirilloxanthin series as photosynthetic pigments. C18:1ω7c, C18:1ω7c 11-methyl and C16:0 were the major fatty acids of all strains. Diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), ornithine lipid (OL), an unidentified phospholipid (PL), and an unidentified aminolipid (AL) were the major polar lipids of all the strains. According to 16S rRNA gene sequences, all strains clustered phylogenetically with the only species of the genus Rhodothalassium (99.8–99.3% sequence similarity) but only strains JA325 and JA563 were distinctly related (60 + 1.5% DNA–DNA hybridization [DDH]) to the type strain Rhodothalassium salexigens DSM 2132T. However, the genotypic data of strains JA325 and JA563 was not supported because of a large number of phenotypic differences compared to the type strain, therefore, it is proposed that all five newly isolated strains were R. salexigens-like strains. In addition, phylogenetically, the Rhodothalassium clade represented a distinct lineage and formed a deep branch with less than 90% 16S rRNA gene sequence similarity to other orders of the Alphaproteobacteria, and characteristic phenotypic properties also distinguished these bacteria from other purple non-sulfur bacteria. Therefore, the novel family Rhodothalassiaceae fam. nov. and the novel order Rhodothalassiales ord. nov. are proposed for the distinct phyletic line represented by the genus Rhodothalassium.

Document Type: Article
Additional Information: WOS:000317156800005
Keywords: Rhodothalassium, sp. nov., fam. nov., ord. nov; MARINE BACTERIUM; PHOTOTROPHIC ALPHAPROTEOBACTERIUM; RHODOSPIRILLUM-SALEXIGENS; RHODOBACTER-SPHAEROIDES; DEOXYRIBONUCLEIC-ACID; BACTERIOCHLOROPHYLL-B; ALPHA-PROTEOBACTERIA; SPECIES DEFINITION; COMB. NOV; SP. NOV.
Research affiliation: OceanRep > GEOMAR > FB3 Marine Ecology > FB3-MI Marine Microbiology
OceanRep > The Future Ocean - Cluster of Excellence
Refereed: Yes
DOI etc.: 10.1016/j.syapm.2012.09.003
ISSN: 0723-2020
Projects: Future Ocean
Date Deposited: 03 Apr 2013 11:41
Last Modified: 17 Jun 2016 09:37
URI: http://eprints.uni-kiel.de/id/eprint/20823

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