Influence of Temperature, Hypercapnia, and Development on the Relative Expression of Different Hemocyanin Isoforms in the Common CuttlefishSepia officinalis

Strobel, Anneli, Hu, Marian Yong-An, Gutowska, Magdalena, Lieb, Bernhard, Lucassen, Magnus, Melzner, Frank, Pörtner, Hans O. and Mark, Felix C. (2012) Influence of Temperature, Hypercapnia, and Development on the Relative Expression of Different Hemocyanin Isoforms in the Common CuttlefishSepia officinalis Journal of Experimental Zoology Part A: Ecological Genetics and Physiology, 317 (8). pp. 511-523. DOI 10.1002/jez.1743.

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Abstract

The cuttlefish Sepia officinalis expresses several hemocyanin isoforms with potentially different pH optima, indicating their reliance on efficient pH regulation in the blood. Ongoing ocean warming and acidification could influence the oxygen-binding properties of respiratory pigments in ectothermic marine invertebrates. This study examined whether S. officinalis differentially expresses individual hemocyanin isoforms to maintain optimal oxygen transport during development and acclimation to elevated seawater pCO2 and temperature. Using quantitative PCR, we measured relative mRNA expression levels of three different hemocyanin isoforms in several ontogenetic stages (embryos, hatchlings, juveniles, and adults), under different temperatures and elevated seawater pCO2. Our results indicate moderately altered hemocyanin expression in all embryonic stages acclimated to higher pCO2, while hemocyanin expression in hatchlings and juveniles remained unaffected. During the course of development, total hemocyanin expression increased independently of pCO2 or thermal acclimation status. Expression of isoform 3 is reported for the first time in a cephalopod in this study and was found to be generally low but highest in the embryonic stages (0.2% of total expression). Despite variable hemocyanin expression, hemolymph total protein concentrations remained constant in the experimental groups. Our data provide first evidence that ontogeny has a stronger influence on hemocyanin isoform expression than the environmental conditions chosen, and they suggest that hemocyanin protein abundance in response to thermal acclimation is regulated by post-transcriptional/translational rather than by transcriptional modifications

Document Type: Article
Keywords: HEAVY-CHAIN ISOFORMS; ACID-BASE REGULATION; GENE-EXPRESSION; OCEAN ACIDIFICATION; THERMAL TOLERANCE; HALIOTIS-TUBERCULATA; LARVAL DEVELOPMENT; PHYSIOLOGICAL-BASIS; PROTEIN-SYNTHESIS; CANCER-MAGISTER
Research affiliation: Kiel University
OceanRep > GEOMAR > FB3 Marine Ecology > FB3-EOE-B Experimental Ecology - Benthic Ecology
Refereed: Yes
DOI etc.: 10.1002/jez.1743
ISSN: 1932-5223
Date Deposited: 09 Nov 2012 08:28
Last Modified: 11 Jul 2017 09:26
URI: http://eprints.uni-kiel.de/id/eprint/19147

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