Comparison of tetrahydrofuran, fetal calf serum, and Tween 40 for the delivery of astaxanthin and canthaxanthin to HepG2 cells

Boesch-Saadatmandi, C., Rimbach, G., Jungblut, A. and Frank, J. (2011) Comparison of tetrahydrofuran, fetal calf serum, and Tween 40 for the delivery of astaxanthin and canthaxanthin to HepG2 cells Cytotechnology, 63 (1). pp. 89-97. DOI 10.1007/s10616-010-9324-7.

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Supplementary data:

Abstract

The present investigation aimed to compare fetal calf serum (FCS) and Tween 40 with the commonly employed tetrahydrofuran (THF) with respect to cytotoxicity, stability of the solubilized carotenoids, and uptake and accumulation of the xanthophylls astaxanthin (AX) and canthaxanthin (CX) in cultured human liver cells (HepG2). Incubation of HepG2 cells for 24 h with THF (a parts per thousand yen1.25) or FCS (a parts per thousand yen11.25) with or without AX (a parts per thousand yen25 mu mol/L) or CX (a parts per thousand yen25 mu mol/L) did not affect cell viability. Tween 40 (0.25-1.25 in medium) reduced cell viability by 75-99. The stabilities of AX and CX in cell-free RPMI 1640 medium for a parts per thousand currency sign24 h were higher when delivered with THF instead of FCS. The dose- and time-dependent accumulations of AX and CX (1-10 mu mol/L) in HepG2 cells were higher when carotenoids were delivered with FCS compared to THF. In conclusion, FCS and THF, but not Tween 40, were suitable solvent systems for the delivery of AX and CX to HepG2 cells. In our experiments FCS was superior with regard to the uptake and accumulation of both carotenoids.

Document Type: Article
Keywords: Carotenoids Astaxanthin Canthaxanthin Delivery vehicle HepG2 cell culture Fetal calf serum Tween 40 Tetrahydrofuran beta-carotene alpha-tocopherol intestinal-absorption cultured-cells human liver vitamin-a in-vitro plasma lycopene vehicle
Research affiliation: Kiel University
Refereed: Yes
DOI etc.: 10.1007/s10616-010-9324-7
ISSN: 0920-9069
Date Deposited: 01 Nov 2012 05:05
Last Modified: 23 Jan 2013 10:01
URI: http://eprints.uni-kiel.de/id/eprint/16838

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