Effect of quercetin and its metabolites isorhamnetin and quercetin-3-glucuronide on inflammatory gene expression: role of miR-155

Boesch-Saadatmandi, C., Loboda, A., Wagner, A. E., Stachurska, A., Jozkowicz, A., Dulak, J., Doring, F., Wolffram, S. and Rimbach, G. (2011) Effect of quercetin and its metabolites isorhamnetin and quercetin-3-glucuronide on inflammatory gene expression: role of miR-155 Journal of Nutritional Biochemistry, 22 (3). pp. 293-299. DOI 10.1016/j.jnutbio.2010.02.008.

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Abstract

In the present study the effect of quercetin and its major metabolites quercetin-3-glucuronide (Q3G) and isorhamnetin on inflammatory gene expression was determined in murine RAW264.7 macrophages stimulated with lipopolysaccharide. Quercetin and isorhamnetin but not Q3G significantly decreased mRNA and protein levels of tumor necrosis factor alpha. Furthermore a significant decrease in mRNA levels of interleukin 13, interleukin 6, macrophage inflammatory protein 1 alpha and inducible nitric oxide synthase was evident in response to the quercetin treatment. However Q3G did not affect inflammatory gene expression. Anti-inflammatory properties of quercetin and isorhamnetin were accompanied by an increase in heme oxygenase 1 protein levels, a downstream target of the transcription factor Nrf2, known to antagonize chronic inflammation. Furthermore, proinflammatory microRNA-155 was down-regulated by quercetin and isorhamnetin but not by Q3G. Finally, anti-inflammatory properties of quercetin were confirmed in vivo in mice fed quercetin-enriched diets (0.1 mg quercetin/g diet) over 6 weeks. (C) 2011 Elsevier Inc. All rights reserved.

Document Type: Article
Keywords: Quercetin Macrophage Inflammation Flavonoid metabolism MicroRNA adhesion molecule expression kappa-b activity flavonoid quercetin endothelial-cells in-vitro platelet-aggregation dietary flavonoids beta-glucuronidase apoe genotype pathway
Research affiliation: Kiel University
Refereed: Yes
DOI etc.: 10.1016/j.jnutbio.2010.02.008
ISSN: 0955-2863
Date Deposited: 01 Nov 2012 05:04
Last Modified: 23 Jan 2013 10:02
URI: http://eprints.uni-kiel.de/id/eprint/16836

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