Mechanical and Metabolic Injury to the Skin Barrier Leads to Increased Expression of Murine beta-Defensin-1, -3, and -14

Ahrens, K., Schunck, M., Podda, G. F., Meingassner, J., Stuetz, A., Schroder, J. M., Harder, J. and Proksch, E. (2011) Mechanical and Metabolic Injury to the Skin Barrier Leads to Increased Expression of Murine beta-Defensin-1, -3, and -14 Journal of Investigative Dermatology, 131 (2). pp. 443-452. DOI 10.1038/jid.2010.289.

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Supplementary data:

Abstract

Protection of the skin against microbiological infection is provided by the permeability barrier and by antimicrobial proteins. We asked whether the expression of murine beta-defensins (mBDs)-1, -3, and -14-orthologs of human beta-defensins hBD-1, -2, and -3, respectively-is stimulated by mechanically/physicochemically (tape stripping or acetone treatment) or metabolically (essential fatty acid-deficient (EFAD) diet) induced skin barrier dysfunction. Both methods led to a moderate induction of mBD-1 and mBD-14 and a pronounced induction of mBD-3 mRNA. Protein expression of the mBDs was increased as shown by immunohistology and by western blotting. Artificial barrier repair by occlusion significantly reduced the increased expression of mBD-14 after mechanical injury and of all three mBDs in EFAD mice, supporting an interrelationship between permeability and the antimicrobial barrier. mBD-3 expression was stimulated in vitro by tumor necrosis factor-alpha (TNF-alpha), and a neutralizing anti-TNF-alpha antibody significantly reduced increased mBD-3 expression after barrier injury in mouse skin, indicating that induction of mBD-3 expression is mediated by cytokines. The expression of mBD-14 was stimulated by transforming growth factor-alpha and not by TNF-alpha. In summary, we demonstrated upregulation of mBD1, -3, and -14 after mechanically and metabolically induced skin barrier disruption, which may be an attempt to increase defense in the case of permeability barrier dysfunction.

Document Type: Article
Keywords: beta-defensin expression fatty acid-deficient antimicrobial peptides permeability barrier atopic-dermatitis epidermal permeability reductase-activity inducible peptide factor receptor mouse
Refereed: Yes
DOI etc.: 10.1038/jid.2010.289
ISSN: 0022-202X
Date Deposited: 01 Nov 2012 05:03
Last Modified: 01 Nov 2012 05:03
URI: http://eprints.uni-kiel.de/id/eprint/16616

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