Conservation and divergence of autonomous pathway genes in the flowering regulatory network of Beta vulgaris

Abou-Elwafa, S. F., Buttner, B., Chia, T., Schulze-Buxloh, G., Hohmann, U., Mutasa-Gottgens, E., Jung, C. and Muller, A. E. (2011) Conservation and divergence of autonomous pathway genes in the flowering regulatory network of Beta vulgaris Journal of Experimental Botany, 62 (10). pp. 3359-3374. DOI 10.1093/jxb/erq321.

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Abstract

The transition from vegetative growth to reproductive development is a complex process that requires an integrated response to multiple environmental cues and endogenous signals. In Arabidopsis thaliana, which has a facultative requirement for vernalization and long days, the genes of the autonomous pathway function as floral promoters by repressing the central repressor and vernalization-regulatory gene FLC. Environmental regulation by seasonal changes in daylength is under control of the photoperiod pathway and its key gene CO. The root and leaf crop species Beta vulgaris in the caryophyllid clade of core eudicots, which is only very distantly related to Arabidopsis, is an obligate long-day plant and includes forms with or without vernalization requirement. FLC and CO homologues with related functions in beet have been identified, but the presence of autonomous pathway genes which function in parallel to the vernalization and photoperiod pathways has not yet been reported. Here, this begins to be addressed by the identification and genetic mapping of full-length homologues of the RNA-regulatory gene FLK and the chromatin-regulatory genes FVE, LD, and LDL1. When overexpressed in A. thaliana, BvFLK accelerates bolting in the Col-0 background and fully complements the late-bolting phenotype of an flk mutant through repression of FLC. In contrast, complementation analysis of BvFVE1 and the presence of a putative paralogue in beet suggest evolutionary divergence of FVE homologues. It is further shown that BvFVE1, unlike FVE in Arabidopsis, is under circadian clock control. Together, the data provide first evidence for evolutionary conservation of components of the autonomous pathway in B. vulgaris, while also suggesting divergence or subfunctionalization of one gene. The results are likely to be of broader relevance because B. vulgaris expands the spectrum of evolutionarily diverse species which are subject to differential developmental and/or environmental regulation of floral transition.

Document Type: Article
Keywords: Autonomous pathway circadian clock FLK floral transition FVE sugar beet floral-promotion pathway rna-binding proteins pre-messenger-rna arabidopsis-thaliana locus-c sugar-beet circadian clock histone-chaperone physical map annual habit
Research affiliation: Kiel University > Faculty of Agricultural and Nutritional Sciences > Institute of Crop Science and Plant Breeding
Refereed: Yes
DOI etc.: 10.1093/jxb/erq321
ISSN: 0022-0957
Date Deposited: 01 Nov 2012 05:05
Last Modified: 23 Jan 2013 10:05
URI: http://eprints.uni-kiel.de/id/eprint/16601

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