Stetteria hydrogenophila, gen. nov. and sp. nov., a novel mixotrophic sulfur-dependent crenarchaeote isolated from Milos, Greece

Jochimsen, B., Peinemann-Simon, S., Volker, H., Stuben, D., Botz, R., Stoffers, P., Dando, P. R. and Thomm, M. (1997) Stetteria hydrogenophila, gen. nov. and sp. nov., a novel mixotrophic sulfur-dependent crenarchaeote isolated from Milos, Greece Extremophiles, 1 (2). pp. 67-73.

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Abstract

A new hyperthermophilic, strictly anaerobic crenarchaeote, Stetteria hydrogenophila DSM11227 representing a new genus within the family of Desulfurococcaceae, was isolated from the sediment of a marine hydrothermal system at Paleohori Bay in Milos, Greece. Cells are gram-negative irregular and disc-shaped cocci, 0.5-1.5 microm in diameter, which are flagellate and can form cytoplasmatic protrusions up to 2 microm in length. The strain grew optimally at 95 degrees C at pH 6.0 and at a NaCl concentration of 3%. The organism grew mixotrophically on peptide substrates. It required elemental sulfur as an external electron acceptor, and in addition, its growth was completely dependent on the presence of molecular hydrogen. Sulfur could be replaced by thiosulfate. H2S, CO2, acetate, and ethanol were identified as products of metabolism. The G + C content of DNA was 65 mol%. Analysis of its phylogenetic position by sequence analysis of 16S rRNA placed this organism in the family of Desulfurococcaceae. The dependence of this organism on both hydrogen and sulfur during growth on peptide substrates distinguishes Stetteria from all previously described species of Crenarchaeota.

Document Type: Article
Additional Information: Jochimsen, B Peinemann-Simon, S Volker, H Stuben, D Botz, R Stoffers, P Dando, P R Thomm, M Research Support, Non-U.S. Gov't Germany Extremophiles : life under extreme conditions Extremophiles. 1997 May;1(2):67-73.
Keywords: Desulfurococcaceae/genetics/growth & development/*isolation & purification/metabolism Greece Hydrogen/*metabolism Microscopy, Electron Phylogeny Sulfur/*metabolism *Water Microbiology
Research affiliation: Kiel University
Refereed: No
ISSN: 1431-0651 (Print) 1431-0651 (Linking)
Date Deposited: 02 Feb 2012 07:59
Last Modified: 08 Oct 2012 10:42
URI: http://eprints.uni-kiel.de/id/eprint/16381

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