Sphaerisporangium flaviroseum sp. nov. and Sphaerisporangium album sp. nov., isolated from forest soil in China

Cao, Y.-R., Jiang, Yi, Xu, L.-H. and Jiang, C.-L. (2009) Sphaerisporangium flaviroseum sp. nov. and Sphaerisporangium album sp. nov., isolated from forest soil in China International Journal of Systematic and Evolutionary Microbiology, 59 (7). pp. 1679-1684. DOI 10.1099/ijs.0.006130-0.

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Abstract

Two Gram-positive, aerobic actinomycete strains, designated YIM 48771(T) and YIM 48782(T), were isolated from virgin forest soil samples collected in Hunan Province, China. 16S rRNA gene sequence similarities of the two novel isolates ranged from 96.3 to 97.6% with species of the genus Sphaerisporangium with validly published names but, in the tree based on 16S rRNA gene sequences, the isolates formed distinct phyletic lines. The level of 16S rRNA gene sequence similarity between the two novel isolates was 97.1%. DNA-DNA hybridization of strains YIM 48771(T) and YIM 48782(T) with recognized species of the genus Sphaerisporangium revealed that the level of DNA-DNA relatedness was below 70%. The DNA G+C contents of strains YIM 48771(T) and YIM 48782(T) were 67.1 and 71 mol%, respectively. Chemotaxonomic data [major menaquinone, MK-9(H(4)); major polar lipids, diphosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylethanolamine and phosphoglycolipids; major fatty acids, iso-C(16:0) and 10-methyl C(17:0)] supported the affiliation of the two isolates with the genus Sphaerisporangium. The results of DNA-DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of the two isolates from recognized Sphaerisporangium species. Based on morphological, chemotaxonomical and phylogenetic data, strains YIM 48771(T) and YIM 48782(T) are considered to represent two novel species of the genus Sphaerisporangium, for which the names Sphaerisporangium flaviroseum sp. nov. (type strain, YIM 48771(T)=DSM 45170(T)=KCTC 19393(T)) and Sphaerisporangium album sp. nov. (type strain, YIM 48782(T)=DSM 45172(T)=CCTCC AA 208026(T)) are proposed.

Document Type: Article
Additional Information: The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains YIM 48771T and YIM 48782T are EU499338 and EU499344, respectively. An extended phylogenetic tree based on 16S rRNA gene sequences, constructed using NJ and ML, showing the relationships of strains YIM 48771T and YIM 48782T and representative species of genera of the family Streptosporangiaceae is available as supplementary material with the online version of this paper.
Keywords: Microbiology; PERFORMANCE LIQUID-CHROMATOGRAPHY; DEOXYRIBONUCLEIC-ACID; CORYNEFORM BACTERIA; SEQUENCE ALIGNMENT; IDENTIFICATION; MP, maximum-parsimony, NJ, neighbour-joining, ML, maximum-likelihood
Research affiliation: OceanRep > GEOMAR > FB3 Marine Ecology > FB3-MI Marine Microbiology
Refereed: Yes
DOI etc.: 10.1099/ijs.0.006130-0
ISSN: 1466-5026
Date Deposited: 10 Jan 2012 08:36
Last Modified: 30 May 2017 08:35
URI: http://eprints.uni-kiel.de/id/eprint/13332

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