Selective enrichment of Green Sulfur Bacteria in the presence of 4- Amino benzenesulfonate (Sulfanilate)

Anil Kumar, P., Srinivas, T. N. R., Sasikala, Ch., Ramana, Ch.V., Süling, Jörg and Imhoff, Johannes F. (2007) Selective enrichment of Green Sulfur Bacteria in the presence of 4- Amino benzenesulfonate (Sulfanilate) World Journal of Microbiology & Biotechnology, 23 (3). pp. 393-399. DOI 10.1007/s11274-006-9237-y.

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A novel selective enrichment method is described for phototrophic green sulfur bacteria even in the presence of purple sulfur and purple nonsulfur bacteria using sulfanilate, which was discovered during efforts to selectively isolate sulfanilate-metabolizing anoxygenic phototrophic bacteria from marine habitats. Samples for these experiments were obtained from beaches, saltpans, subsurface mangrove soils, fish and prawn aquaculture ponds and backwaters of the East and West coasts of India. Photoorganoheterotrophic and photolithoautotrophic enrichments in the absence of sulfanilate predominantly yielded purple bacterial enrichments. In contrast, photolithoautotrophic enrichments in the presence of sulfanilate yielded green-colored enrichments from the same samples. Whole cell absorption spectra of the enrichment cultures revealed the presence of bacteriochlorophyll c and thus green phototrophic bacteria. Microscopic observation demonstrated the presence of sulfur globules outside the bacterial cells and the presence of non-motile cells, some of which had prosthecae. 16S rDNA sequences obtained from green sulfur bacterial strains isolated from enrichment cultures confirmed the presence of representatives of the green sulfur bacterial genera Prosthecochloris and Chlorobaculum. The selective pressure of sulfanilate exerted through inhibition of phototrophic purple sulfur bacteria was demonstrated by inhibition studies using the purple sulfur bacteria Marichromatium indicum JA100 and Marichromatium sp. JA120 (JCM 13533) and the green sulfur bacterium Prosthecochloris sp. JAGS6 (JCM 13299).

Document Type: Article
Keywords: Anoxygenic phototrophic bacteria Green sulfur bacteria Enrichment cultures Aromatic hydrocarbon Sulfanilate
Research affiliation: OceanRep > GEOMAR > FB3 Marine Ecology > FB3-MI Marine Microbiology
OceanRep > The Future Ocean - Cluster of Excellence
Refereed: Yes
DOI etc.: 10.1007/s11274-006-9237-y
ISSN: 0959-3993
Projects: Future Ocean
Date Deposited: 16 Feb 2010 12:28
Last Modified: 26 Apr 2016 07:46

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